Detects human FGF-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FGF-4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -18, -19, -20, -21, -23, rhFGF acidic, rhFGF basic, recombinant mouse (rm) FGF-8b, -8c, or -15 is observed.
Monoclonal Mouse IgG2A Clone # 254625
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human FGF-3 Asp28-Arg212 Accession # P11487
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Immersion fixed paraffin-embedded sections of human breast cancer tissue
Measured by its ability to neutralize FGF‑3-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 2-5 µg/mL in the presence of 0.3 µg/mL Recombinant Human FGF‑3 and 1 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF‑3 and Neutralization by Human FGF‑3 Antibody. Recombinant Human FGF‑3 (Catalog # 1206-F3) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑3 (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF‑3 Monoclonal Antibody (Catalog # MAB1206). The ND50 is typically 2-5 µg/mL in the presence of heparin (1 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibroblast Growth Factor 3 (FGF-3) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4).
The FGF-3 gene, originally designated int-2, was first identified as a proto-oncogene activated in mouse mammary tumors by proviral integration (2). Amplification of this gene has also been found frequently in human tumors. Human FGF-3 cDNA predicts a 239 aa precursor protein with a 17 aa signal peptide and a 222 aa secreted mature protein with one potential N-linked glycosylation site (1). Human and mouse FGF-3 share 88% aa sequence identity. The Xenopus and mammalian secreted FGF-3 are processed proteolytically at both the N- and C-terminus (5). FGF-3 binds with high-affinity to the IIIb isoforms of FGF R1 and FGF R2. FGF-3 also binds the IIIc isoform of FGF R2, but with lower affinity (6). FGF-3 has been implicated in the induction of inner ear development (7). Studies have suggested that FGF-3 and FGF-8 function synergistically in otic placode induction and during neuronal development to regulate dorsoventral axis formation (8 - 10). During development, the activities of FGF-3 and FGF-8 are regulated negatively by the sprouty family proteins and by Sef (similar expression to fgf genes), a transmebrane protein that shares intracellular sequence similarities with the IL-17 receptor (10).
Brookes, S. et al. (1989) Oncogene 4:429.
Dickson, C. et al. (1989) Prog. Growth Factor Res. 1:123.
Goldfarb, M. (1996) Cytokine and Growth Factor Reviews 7:311.
Green, P. et al. (1996) BioEssays 18:639.
Antoine, M. et al. (2000) Cell Growth Differen. 11:593.
Kohl, R. et al. (2002) J. Biol. Chem. 277:32760.
Represa, J. et al. (1991) Nature 353:561.
Maroon, H. et al. (2002) Development, 129:2099.
Walshe, J. et al. (2002) Current Biol. 12:1117.
Furthauer, M. et al. (2002) Nature Cell Biol. 4:170.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.