Human FGF-9 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF273
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Human FGF-9 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-9 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-16, rhFGF acidic and rhFGF basic is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF-9 (R&D Systems, Catalog # 273-F9)
Met1-Ser208
Accession # P31371
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FGF‑9 (Catalog # 273-F9)

Human FGF-9 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human FGF‑9 Antibody (Catalog # MAB273)

Standard: Recombinant Human FGF-9 Protein (Catalog # 273-F9)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-9

The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal and epithelial origin. All FGFs have two conserved cysteine residues and share 30 - 50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells.

The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.

References
  1. Naruo, K. et al. (1993) J. Biol. Chem. 268:2857.
  2. Miyamoto, M. et al. (1993) Mol. Cell Biol. 13:4251.
Long Name
Fibroblast Growth Factor 9
Entrez Gene IDs
2254 (Human); 14180 (Mouse)
Alternate Names
FGF9; FGF-9; fibroblast growth factor 9 (glia-activating factor); Fibroblast growth factor 9; GAF; glia-activating factor; HBFG-9; HBGF-9; Heparin-binding growth factor 9; MGC119914; MGC119915; SYNS3

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