Human FGF-9 Antibody Summary
Accession # P31371
Human FGF-9 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF‑9 and Neutralization by Human FGF‑9 Antibody. Recombinant Human FGF-9 (Catalog # 273-F9) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-9 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF-9 Monoclonal Antibody (Catalog # MAB273). The ND50 is typically 0.1-0.6 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal and epithelial origin. All FGFs have two conserved cysteine residues and share 30‑50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells.
The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.
- Naruo, K. et al. (1993) J. Biol. Chem. 268:2857.
- Miyamoto, M. et al. (1993) Mol. Cell Biol. 13:4251.
Citations for Human FGF-9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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FGF9 from cancer-associated fibroblasts is a possible mediator of invasion and anti-apoptosis of gastric cancer cells.
Authors: Sun C, Fukui H, Hara K, Zhang X, Kitayama Y, Eda H, Tomita T, Oshima T, Kikuchi S, Watari J, Sasako M, Miwa H
BMC Cancer, 2015;15(0):333.
Sample Types: Whole Cells
Fibroblast growth factor 9 has oncogenic activity and is a downstream target of Wnt signaling in ovarian endometrioid adenocarcinomas.
Authors: Hendrix ND, Wu R, Kuick R, Schwartz DR, Fearon ER, Cho KR
Cancer Res., 2006;66(3):1354-62.
Sample Types: Cell Lysates
Applications: Western Blot
Expression and possible function of fibroblast growth factor 9 (FGF9) and its cognate receptors FGFR2 and FGFR3 in postnatal and adult retina.
Authors: Cinaroglu A, Ozmen Y, Ozdemir A, Ozcan F, Ergorul C, Cayirlioglu P, Hicks D, Bugra K
J. Neurosci. Res., 2005;79(3):329-39.
Sample Types: Whole Cells
Fgf9 induces proliferation and nuclear localization of FGFR2 in Sertoli precursors during male sex determination.
Authors: Schmahl J, Kim Y, Colvin JS, Ornitz DM, Capel B
Sample Types: Recombinant Protein
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