Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

R&D Systems | Catalog # HSFB00D

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Key Product Details

Assay Length

5.5 hours

Sample Type & Volume Required Per Well

Serum (100 µL), EDTA Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)

Sensitivity

0.07 pg/mL

Assay Range

0.313-20 pg/mL (Serum, EDTA Plasma, Citrate Plasma, Urine)
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Product Summary for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

The Quantikine HS Human FGF basic Immunoassay kit is a 5.5 hour solid phase ELISA designed to measure FGF basic levels in serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450 or 490nm (TMB or AP Substrate)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human FGF basic

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Citrate Plasma, EDTA Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 1.31 5.32 11.4 1.51 5.71 11.1
Standard Deviation 0.10 0.23 0.40 0.12 0.27 0.91
CV% 7.6 4.3 3.5 7.9 4.7 8.2

Recovery for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

The recovery of FGF basic spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Citrate Plasma (n=4) 97 83-118
EDTA Plasma (n=4) 99 85-109
Serum (n=4) 100 88-116
Urine (n=4) 109 100-120

Linearity

To assess the linearity of the assay, the following biological samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and assayed.

Human FGF basic ELISA Linearity

Scientific Data Images for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

Human FGF basic ELISA Standard Curve

Human FGF basic ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Gene Symbol

FGF2

Additional FGF basic/FGF2/bFGF Products

Product Documents for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

For research use only

Citations for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit

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