FGF basic, also known as FGF-2 and HBGF-2, is a member of the FGF family of mitogenic proteins. FGF basic is widely expressed and affects diverse aspects of embryonic development by regulating cell proliferation, differentiation, and survival. Various N-terminal extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. Mature human FGF basic shares greater than 96% aa sequence identity with bovine, mouse, and rat FGF basic.
Human FGF basic/FGF2/bFGF Antibody (10043) [Biotin]
R&D Systems | Catalog # BAM233
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Microarray Development
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2A Clone # 10043
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Product Specifications
Immunogen
E. coli-derived recombinant human FGF basic/FGF2/bFGF
Specificity
Detects human FGF basic/FGF2/bFGF in ELISAs. In ELISAs, this antibody also recognizes bovine FGF basic but not bovine FGF acidic, recombinant human (rh) FGF acidic, rhFGF‑4, rhFGF‑6, or rhFGF‑7.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human FGF basic/FGF2/bFGF Antibody (10043) [Biotin]
Human FGF basic ELISA Standard Curve
Human CD117/c-kit was serially diluted and captured by Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB233) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human FGF basic/FGF2/bFGF Biotinylated Monoclonal Antibody (Catalog # BAM233) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human FGF basic/FGF2/bFGF Antibody (10043) [Biotin]
Human FGF basic/FGF2/bFGF Sandwich Immunoassay
ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
Use in combination with these reagents:
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using BAM233 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FGF basic/FGF2/bFGF
Long Name
Fibroblast Growth Factor basic
Alternate Names
bFGF, FGF-2, FGF2, HBGF-2, Prostatropin
Gene Symbol
FGF2
Additional FGF basic/FGF2/bFGF Products
Product Documents for Human FGF basic/FGF2/bFGF Antibody (10043) [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human FGF basic/FGF2/bFGF Antibody (10043) [Biotin]
For research use only
Related Research Areas
Citations for Human FGF basic/FGF2/bFGF Antibody (10043) [Biotin]
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/16/2017The biotinylated antibody BAM233 was used as a detection antibody with MAB233 as the capture in an ELISA.
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