Human FGF basic/FGF2 Quantikine HS ELISA Kit

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PHSFB00D
SSFB00D
HSFB00D
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FGF basic/FGF2 ELISA Kit ELISA
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Citations (16)
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Human FGF basic/FGF2 Quantikine HS ELISA Kit Summary

Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Sensitivity
0.07 pg/mL
Assay Range
0.3 - 20 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human FGF basic
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine HS Human FGF basic Immunoassay kit is a 5.5 hour solid phase ELISA designed to measure FGF basic levels in serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1.31 5.32 11.4 1.51 5.71 11.1
Standard Deviation 0.1 0.23 0.4 0.12 0.27 0.91
CV% 7.7 4.4 3.5 8.2 4.7 8.2

Recovery

The recovery of FGF basic spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Citrate Plasma (n=4) 97 83-118
EDTA Plasma (n=4) 99 85-109
Serum (n=4) 100 88-116
Urine (n=4) 109 100-120

Linearity

To assess the linearity of the assay, the following biological samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and assayed.
Human FGF basic ELISA Linearity

Data Examples

Human FGF basic ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF basic/FGF2

FGF basic/FGF2 is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic binds to FGF R1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name:
Fibroblast Growth Factor basic
Entrez Gene IDs:
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names:
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin

Citations for Human FGF basic/FGF2 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Dose selection trial of metronomic oral vinorelbine monotherapy in patients with metastatic cancer: a hellenic cooperative oncology group clinical translational study.
    Authors: Briasoulis, Evangelo, Aravantinos, Gerasimo, Kouvatseas, George, Pappas, Periklis, Biziota, Eirini, Sainis, Ioannis, Makatsoris, Thomas, Varthalitis, Ioannis, Xanthakis, Ioannis, Vassias, Antonios, Klouvas, George, Boukovinas, Ioannis, Fountzilas, George, Syrigos, Kostanti, Kalofonos, Haralamb, Samantas, Epaminon
    BMC Cancer, 2013;13(1):263.
    Species: Human
    Sample Types: Plasma
  2. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
    Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
    Clin. Cancer Res., 2012;18(8):2290-300.
    Species: Human
    Sample Types: Plasma
  3. Local cytokine concentrations and oxygen pressure are related to maturation of the collateral circulation in humans.
    Authors: Schirmer SH, van Royen N, Moerland PD, Fledderus JO, Henriques JP, van der Schaaf RJ, Vis MM, Baan J, Koch KT, Horrevoets AJ, Hoefer IE, Piek JJ
    J. Am. Coll. Cardiol., 2009;53(23):2141-7.
    Species: Human
    Sample Types: Plasma
  4. Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates.
    Authors: Kanter J, Khan SY, Kelher M, Gore L, Silliman CC
    Clin. Cancer Res., 2008;14(12):3942-7.
    Species: Human
    Sample Types: Platelet-Poor Plasma
  5. Fibroblast growth factor-2 expression is altered in lambs with increased pulmonary blood flow and pulmonary hypertension.
    Authors: Wedgwood S, Devol JM, Grobe A, Benavidez E, Azakie A, Fineman JR, Black SM
    Pediatr. Res., 2007;61(1):32-6.
    Species: Ovine
    Sample Types: Serum
  6. Dedicated epithelial recipient cells determine pigmentation patterns.
    Authors: Weiner L, Han R, Scicchitano BM, Li J, Hasegawa K, Grossi M, Lee D, Brissette JL
    Cell, 2007;130(5):932-42.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
    Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
    Br. J. Haematol., 2007;137(6):560-8.
    Species: Human
    Sample Types: Serum
  8. Endostatin variations in childhood acute lymphoblastic leukaemia--comparison with basic fibroblast growth factor and vascular endothelial growth factor.
    Authors: Schneider P, Vasse M, Corbiere C, Legrand E, Marie-Cardine A, Boquet C, Cazin L, Vannier JP
    Leuk. Res., 2007;31(5):629-38.
    Species: Human
    Sample Types: Plasma
  9. Elevated levels of fibroblast growth factor-2 in serum from women with endometriosis.
    Authors: Bourlev V, Larsson A, Olovsson M
    Am. J. Obstet. Gynecol., 2006;194(3):755-9.
    Species: Human
    Sample Types: Peritoneal Fluid
  10. Phase II study of recombinant human endostatin in patients with advanced neuroendocrine tumors.
    Authors: Kulke MH, Bergsland EK, Ryan DP, Enzinger PC, Lynch TJ, Zhu AX, Meyerhardt JA, Heymach JV, Fogler WE, Sidor C, Michelini A, Kinsella K, Venook AP, Fuchs CS
    J. Clin. Oncol., 2006;24(22):3555-61.
    Species: Human
    Sample Types: Urine
  11. Phase I clinical and pharmacokinetic study of PTK/ZK, a multiple VEGF receptor inhibitor, in patients with liver metastases from solid tumours.
    Authors: Mross K, Drevs J, Muller M, Medinger M, Marme D, Hennig J, Morgan B, Lebwohl D, Masson E, Ho YY, Gunther C, Laurent D, Unger C
    Eur. J. Cancer, 2005;41(9):1291-9.
    Species: Human
    Sample Types: Plasma
  12. Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release.
    Authors: Lacoste E, Martineau I, Gagnon G
    J. Periodontol., 2003;74(10):1498-507.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Inhibition of growth and metastasis of orthotopic human prostate cancer in athymic mice by combination therapy with pegylated interferon-alpha-2b and docetaxel.
    Authors: Huang SF, Kim SJ, Lee AT, Karashima T, Bucana C, Kedar D, Sweeney P, Mian B, Fan D, Shepherd D, Fidler IJ, Dinney CP, Killion JJ
    Cancer Res., 2002;62(20):5720-6.
    Species: Mouse
    Sample Types: Serum
  14. Targeted disruption of fibroblast growth factor receptor-1 blocks maturation of visceral endoderm and cavitation in mouse embryoid bodies.
    Authors: Esner M, Pachernik J, Hampl A, Dvorak P
    Int. J. Dev. Biol., 2002;46(6):817-25.
    Species: Bovine
    Sample Types: Serum
  15. High urine concentrations of basic fibroblast growth factor in dogs with bladder cancer.
    Authors: Allen DK, Waters DJ, Knapp DW, Kuczek T
    J. Vet. Intern. Med., 1996;10(4):231-4.
    Species: Canine
    Sample Types: Whole Cells
  16. High urine concentrations of basic fibroblast growth factor in dogs with bladder cancer.
    Authors: Allen DK, Waters DJ, Knapp DW, Kuczek T
    J. Vet. Intern. Med., 1996;10(4):231-4.
    Species: Canine
    Sample Types: Urine

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