Human Fibulin 3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Fibulin 3 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Fibulin 3
    Gln19-Phe493
    Accession # Q12805
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Fibulin 3 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and human muscle tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Fibulin 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6235) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibulin 3 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibulin 3

Fibulin 3 (also S1-5 and EFEMP1) is a secreted, monomeric 55‑57 kDa member of the fibulin family of proteins. In mouse, Fibulin 3 appears in an O-glycosylated 63 and 80 kDa form. Given the high amino acid (aa) conservation between mouse and human, human Fibulin 3 may also demonstrate like isoforms. Fibulin 3 is expressed by fibroblasts, endothelial cells and prechondrocytes, and appears to provide both stability to ECM (elastin and basement membrane), and to regulate cell maturation/ differentiation. Fibulin 3 is reported to activate the EGF receptor, an effect that may influence multiple cell types under multiple scenarios. Mature human Fibulin 3 is 476 aa in length. It contains six EGF-like domains (aa 26‑378), at least one of which binds to the EGF receptor and induces EGFR phosphorylation. There is at least one alternative start site at Met107 that generates a 43 kDa short form. Full-length human Fibulin 3 shares 95% aa identity with mouse Fibulin 3.

  • Entrez Gene IDs:
    2202 (Human); 216616 (Mouse); 305604 (Rat)
  • Alternate Names:
    DHRD; EFEMP1; EGF containing fibulin-like extracellular matrix protein 1; EGF-containing fibulin-like extracellular matrix protein 1; Extracellular protein S1-5; FBLN3; FBLN3DRAD; FBNLFLJ35535; FIBL-3; Fibrillin-like protein; fibrillin-like; Fibulin 3; fibulin-3; MGC111353; MLVT; MTLV; S1-5
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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