Human FLRT2 Antibody

Catalog # Availability Size / Price Qty
AF2877
AF2877-SP
Product Details
Citations (4)
FAQs
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Human FLRT2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FLRT2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FLRT2
Cys36-Ser539
Accession # O43155
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FLRT2 (Catalog # 2877-FL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLRT2

FLRT2 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85 kDa type I transmembrane (TM) human FLRT2 is synthesized as a 660 amino acid (aa) precursor with a 35 aa signal sequence, a 506 aa extracellular domain (ECD), a 21 aa TM segment and a 98 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT2 ECD shares 97%, 96%, 99%, 96% and 95% aa sequence identity with mouse, rat, equine, canine and bovine FLRT2 ECD, respectively. Human FLRT1 and FLRT3 ECDs share approximately 47% aa identity with FLRT2. The fibronectin domain of all three FLRTs can bind to FGF receptors (2). This binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). In adulthood, FLRT2 mRNA is most abundant in pancreas, but is also present in skeletal muscle, brain and heart (1). FLRT2 in mouse embryos shows highest expression in a subset of the sclerotome in the brain, the stomach, and posterior to the developing heart (2). This expression is distinct from that of FLRT1 and FLRT3 (2).

References
  1. Lacy, S. E. et al. (1999) Genomics 62:417.
  2. Haines, B. P. et al. (2006) Dev. Biol. 297:14.
  3. Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.
  4. Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
  5. Ogata, S. et al. (2007) Genes Dev. 21:1817.
Long Name
Fibronectin Leucine Rich Transmembrane Protein 2
Entrez Gene IDs
23768 (Human); 399558 (Mouse)
Alternate Names
Fibronectin Leucine Rich Transmembrane Protein 2; Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 2; FLRT2; KIAA0405; Leucine-Rich Repeat Transmembrane Protein FLRT2

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Citations for Human FLRT2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. LPHN2 inhibits vascular permeability by differential control of endothelial cell adhesion
    Authors: Chiara Camillo, Nicola Facchinello, Giulia Villari, Giulia Mana, Noemi Gioelli, Chiara Sandri et al.
    Journal of Cell Biology
  2. Synaptogenic activity of the axon guidance molecule Robo2 underlies hippocampal circuit function
    Authors: H Blockus, SV Rolotti, M Szoboszlay, E Peze-Heids, T Ming, A Schroeder, N Apostolo, KM Vennekens, PS Katsamba, F Bahna, S Mannepalli, G Ahlsen, B Honig, L Shapiro, J de Wit, A Losonczy, F Polleux
    Cell Reports, 2021-10-19;37(3):109828.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Placental labyrinth formation in mice requires endothelial FLRT2-UNC5B signaling
    Authors: I Tai-Nagara, Y Yoshikawa, N Numata, T Ando, K Okabe, Y Sugiura, M Ieda, N Takakura, O Nakagawa, B Zhou, K Okabayashi, M Suematsu, Y Kitagawa, M Bastmeyer, K Sato, R Klein, S Navankasat, DY Li, S Yamagishi, Y Kubota
    Development, 2017-06-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  4. Tumor-specific inter-endothelial adhesion mediated by FLRT2 facilitates cancer aggressiveness
    Authors: Ando T, Tai-Nagara I, Sugiura Y et al.
    The Journal of clinical investigation

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