Human FOLR4 Antibody Summary
Accession # NP_001073955
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FOLR4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FOLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6328) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
FOLR4 (Folate receptor 4; also FR4 and folate receptor δ and folate binding protein 3) is a 34‑36 kDa member of the folate receptor family of molecules. Based on mouse, it appears to be a marker for subsets of T cells. In particular, and in conjunction with CD25, FR4hiCD25hi expression defines T reg cells, FR4loCD25+ expression pattern identifies effector memory T cells, and a FR4hiCD25- combination characterizes central memory T cells. Mature human FOLR4 is 225 amino acids (aa) in length (aa 20‑244). It contains virtually no identifiable motifs. There is one potential splice variant that shows a five aa substitution for aa 111‑116. Over aa 134‑220, human FOLR4 shares 72% aa identity with mouse FOLR4.
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