Human FoxH1 Biotinylated Antibody

Catalog #: BAF4248 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF4248

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxH1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxH1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human FoxB2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FoxH1
Ser164-Ser272
Accession # O75593

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human FoxH1

Immunocytochemistry

5-15 µg/mL

Immersion fixed BG01V human embryonic stem cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxH1

FoxH1 (also known as FAST-1) is a 39 kDa, class 2, subfamily H member of the FOX (forkhead box) family of transcription factors. It is a nuclear protein that binds DNA promoter sites. It does not independently regulate transcription but interacts with SMAD2/4 to activate, and SMAD3/4 to inhibit gene transcription. Human FOXH1 is 365 amino acids (aa) in length. It contains an NLS (aa 22‑30), one FKH (fork head) DNA-binding domain (aa 32‑128) and a SMAD-interaction region (aa 273‑354). Over aa 164‑272, human FOXH1 is 55% identical to mouse FoxH1.

Long Name

Forkhead Box J1

Entrez Gene IDs

8928 (Human)

Alternate Names

FAST1; Fast-1; FAST1forkhead box protein H1; FAST2; Fast-2; Forkhead activin signal transducer 1; Forkhead activin signal transducer 2; forkhead activin signal transducer-1; forkhead box H1; FoxH1; hFAST-1; TGF-beta/activin signal transducer