Human FSH beta Antibody
Human FSH beta Antibody Summary
Accession # P01225
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FSH beta by Western Blot. Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FSH beta Monoclonal Antibody (Catalog # MAB43102) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for FSH beta at approximately 18-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FSH beta
Follicle-stimulating hormone (FSH; also follitropin) is a 33 kDa heterodimer belonging to the glycoprotein hormones subunit beta family (1). The heterodimer is composed of two glycoproteins linked noncovalently: a common alpha subunit, which is also a component of luteinizing hormone, thyroid stimulating hormone, and chorionic gonadotropin; and a unique beta subunit that confers the protein’s specific biological action and is responsible for the interaction with FSH-receptor. The alpha subunit is 20 kDa and is synthesized as a 116 amino acid (aa) precursor that contains a 24 aa signal sequence and a 92 aa mature chain, which contains two potential sites of N-linked glycosylation (1-2). The beta subunit is 23 kDa and is synthesized as a 129 aa precursor with an 18 aa signal sequence and a 111 aa mature chain, which also contains two potential sites of N-linked glycosylation (1). Human FSH beta shares approximately 90% aa sequence identity with mouse and rat FSH beta. FSH is secreted by gonadotropes of the anterior pituitary gland. Its release is controlled by pulses of gonadotropin-releasing hormone (GnRH), and those pulses, in turn, are subject to the estrogen feedback from the gonads. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. It promotes follicle maturation and spermatogenesis through interactions with FSH-receptor.
- Van de Wiel, D.F. et al. (1998) J. Mol. Endocrinol. 20:83.
- Rathnam, P. and B.B. Saxena (1975) J. Biol. Chem. 250:6735.
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