Human Furin APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Furin in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 222722
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Furin
    Accession # NP_002560
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Furin in H9 Human Cell Line by Flow Cytometry. H9 human cutaneous T lymphoma cell line was stained with Mouse Anti-Human Furin APC‑conjugated Monoclonal Antibody (Catalog # IC1503A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Furin

Furin is a member of the Proprotein Convertase (PC) family, which belongs to the subtilisin superfamily of serine proteases (1-3). As a cellular protease, Furin processes a variety of proproteins in secretory pathway compartments by cleaving after Arg-Xaa-Lys/Arg-Arg-like motifs, which usually reside at the end of the pro regions of these proproteins. Examples of the proprotein substrates are growth factors and receptors, extracellular matrix proteins, and other proteases. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax. It is synthesized as a 794 amino acid (aa) type I transmembrane protein precursor with a signal peptide (aa residues 1-24), a pro region (aa residues 25-107 which play a crucial role in the folding, activation and transport of Furin) and a mature chain (aaresidues 108-794). The mature chain consists of the subtilisin-like catalytic domain, a P domain which is essential for enzyme activity and the modulation of pH and calcium requirements, and a cytoplasmic domain which controls the localization and sorting of Furin in the trans-Golgi network/endosomal system. The purified recombinant human Furin (residues 108-715) corresponds to the mature enzyme that terminates before the transmembrane domain. Over aa 108-715, human and mouse Furin share 95% aa sequence identity (1-3).

  • References:
    1. Van den Ouweland, A.M. et al. (1990) Nucleic Acids Res. 18:664.
    2. Barr, P.J. et al. (1991) DNA Cell Biol. 10:319.
    3. Thomas, G. (2002) Nat. Rev. Mol. Cell Biol. 3:753.
  • Entrez Gene IDs:
    5045 (Human); 18550 (Mouse)
  • Alternate Names:
    Dibasic-processing enzyme; EC 3.4.21; EC; FUR; FURdibasic processing enzyme; furin (paired basic amino acid cleaving enzyme); furin, membrane associated receptor protein; PACE; PACEFES upstream region; paired basic amino acid cleaving enzyme (furin, membrane associated receptorprotein); Paired basic amino acid residue-cleaving enzyme; PC; PCSK3; PCSK3furin; proprotein convertase subtilisin/kexin type 3; SPC1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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