Human FXR/NR1H4 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    This antibody specifically recognizes human FXR and cross-reacts with rat and mouse FXR. Not yet tested in other species.
  • Source
    Monoclonal Mouse IgG2A Clone # A9033A
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Recombinant human FXR/NR1H4
    aa 2-126
  • Formulation
    A liquid formulation in physiologic saline with 0.1% NaN3
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    This antibody can be used at 1 μg/mL with the appropriatesecondary reagents to detect human FXR.
  • Immunohistochemistry
    This antibody can be used at 20-40 μg/mL with theappropriate secondary reagents to detect human FXR.
  • Immunoprecipitation
    Optimal dilutions should be determined by eachlaboratory.
  • Direct ELISA
    This antibody can be used at 0.2 μg/mL with the appropriatesecondary reagents to detect human FXR.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Background: FXR/NR1H4
Farnesoid X-activated Receptor (FXR, HRR-1, BAR, RIP14; NR1H4) is a member of the Orphan Nuclear Receptor family. FXR is expressed in the liver, intestinal villi, renal tubes and adrenal cortex. FXR is a global regulator of bile acid metabolism. Two genes, cholesterol 7a-hydroxylase (CYP7A1) and IBABP (Ileal Bile Acid Binding Protein), which are implicated in bile acid biosynthesis and recycling, respectively, are target genes of FXR. FXR is shown to be transcriptionally activated by farnesol metabolites such as farnesol itself and juvenile hormone III. FXR binds to DNA only as a heterodimer with Retinoid X Receptor (RXR).
  • Long Name:
    Farnesoid X-activated receptor
  • Entrez Gene IDs:
    9971 (Human); 20186 (Mouse); 60351 (Rat)
  • Alternate Names:
    BAR; bile acid receptor; farnesoid X receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; FXR; FXRBAR; HRR-1; HRR1MGC163445; NR1H4; Nuclear receptor subfamily 1 group H member 4; nuclear receptor subfamily 1, group H, member 4; Retinoid X receptor-interacting protein 14; RIP14; RIP14RXR-interacting protein 14
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Immunomodulatory glycan LNFPIII alleviates hepatosteatosis and insulin resistance through direct and indirect control of metabolic pathways.
    Nat Med, 2012;18(11):1665-72.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. FXR Controls the Tumor Suppressor NDRG2 and FXR Agonists Reduce Liver Tumor Growth and Metastasis in an Orthotopic Mouse Xenograft Model.
    Authors: Deuschle U, Schuler J, Schulz A, Schluter T, Kinzel O, Abel U, Kremoser C
    PLoS ONE, 2012;7(10):e43044.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Bile acids down-regulate caveolin-1 in esophageal epithelial cells through sterol responsive element-binding protein.
    Authors: Prade E, Tobiasch M, Hitkova I, Schaffer I, Lian F, Xing X, Tanzer M, Rauser S, Walch A, Feith M, Post S, Rocken C, Schmid RM, Ebert MP, Burgermeister E
    Mol. Endocrinol., 2012;26(5):819-32.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Farnesoid X receptor protects human and murine gastric epithelial cells against inflammation-induced damage.
    Authors: Lian F, Xing X, Yuan G, Schafer C, Rauser S, Walch A, Rocken C, Ebeling M, Wright MB, Schmid RM, Ebert MP, Burgermeister E
    Biochem. J., 2011;438(2):315-23.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Farnesoid X receptor, overexpressed in pancreatic cancer with lymph node metastasis promotes cell migration and invasion.
    Authors: Lee JY, Lee KT, Lee JK, Lee KH, Jang KT, Heo JS, Choi SH, Kim Y, Rhee JC
    Br. J. Cancer, 2011;104(6):1027-37.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
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