Human FXR/NR1H4 Antibody

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Product Details
Citations (9)
Supplemental Products
Reviews (1)

Human FXR/NR1H4 Antibody Summary

Species Reactivity
This antibody specifically recognizes human FXR and cross-reacts with rat and mouse FXR. Not yet tested in other species.
Monoclonal Mouse IgG2A Clone # A9033A
Protein A or G purified from hybridoma culture supernatant
Recombinant human FXR/NR1H4
aa 2-126
A liquid formulation in physiologic saline with 0.1% NaN3


Recommended Concentration
Western Blot
This antibody can be used at 1 μg/mL with the appropriatesecondary reagents to detect human FXR.
This antibody can be used at 20-40 μg/mL with theappropriate secondary reagents to detect human FXR.
Optimal dilutions should be determined by eachlaboratory.
Direct ELISA
This antibody can be used at 0.2 μg/mL with the appropriatesecondary reagents to detect human FXR.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: FXR/NR1H4

Farnesoid X-activated Receptor (FXR, HRR-1, BAR, RIP14; NR1H4) is a member of the Orphan Nuclear Receptor family. FXR is expressed in the liver, intestinal villi, renal tubes and adrenal cortex. FXR is a global regulator of bile acid metabolism. Two genes, cholesterol 7a-hydroxylase (CYP7A1) and IBABP (Ileal Bile Acid Binding Protein), which are implicated in bile acid biosynthesis and recycling, respectively, are target genes of FXR. FXR is shown to be transcriptionally activated by farnesol metabolites such as farnesol itself and juvenile hormone III. FXR binds to DNA only as a heterodimer with Retinoid X Receptor (RXR).

Long Name
Farnesoid X-activated receptor
Entrez Gene IDs
9971 (Human); 20186 (Mouse); 60351 (Rat)
Alternate Names
BAR; bile acid receptor; farnesoid X receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; FXR; FXRBAR; HRR-1; HRR1MGC163445; NR1H4; Nuclear receptor subfamily 1 group H member 4; nuclear receptor subfamily 1, group H, member 4; Retinoid X receptor-interacting protein 14; RIP14; RIP14RXR-interacting protein 14

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Citations for Human FXR/NR1H4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Pentacyclic triterpenes modulate farnesoid X receptor expression in colonic epithelial cells: implications for colonic secretory function
    Authors: CM Fallon, JS Smyth, A Quach, N Lajczak-Mc, A O'Toole, KE Barrett, H Sheridan, SJ Keely
    The Journal of Biological Chemistry, 2022;0(0):102569.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. Farnesoid X Receptor Deficiency Induces Hepatic Lipid and Glucose Metabolism Disorder via Regulation of Pyruvate Dehydrogenase Kinase 4
    Authors: W Deng, W Fan, T Tang, H Wan, S Zhao, Y Tan, KA Oware, J Tan, J Li, S Qu
    Oxidative Medicine and Cellular Longevity, 2022;2022(0):3589525.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  3. Farnesoid X receptor as marker of osteotropism of breast cancers through its role in the osteomimetism of tumor cells
    Authors: L Absil, F Journé, D Larsimont, JJ Body, L Tafforeau, D Nonclercq
    BMC Cancer, 2020;20(1):640.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Loss of Thymine DNA Glycosylase Causes Dysregulation of Bile Acid Homeostasis and Hepatocellular Carcinoma
    Authors: HM Hassan, M Isovic, B Kolendowsk, N Bauer-Mais, O Onabote, M Cecchini, A Haig, S Maleki Var, TM Underhill, J Torchia
    Cell Rep, 2020;31(1):107475.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  5. Bile acids down-regulate caveolin-1 in esophageal epithelial cells through sterol responsive element-binding protein.
    Authors: Prade E, Tobiasch M, Hitkova I, Schaffer I, Lian F, Xing X, Tanzer M, Rauser S, Walch A, Feith M, Post S, Rocken C, Schmid RM, Ebert MP, Burgermeister E
    Mol. Endocrinol., 2012;26(5):819-32.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. FXR Controls the Tumor Suppressor NDRG2 and FXR Agonists Reduce Liver Tumor Growth and Metastasis in an Orthotopic Mouse Xenograft Model.
    Authors: Deuschle U, Schuler J, Schulz A, Schluter T, Kinzel O, Abel U, Kremoser C
    PLoS ONE, 2012;7(10):e43044.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Immunomodulatory glycan LNFPIII alleviates hepatosteatosis and insulin resistance through direct and indirect control of metabolic pathways.
    Nat Med, 2012;18(11):1665-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Farnesoid X receptor protects human and murine gastric epithelial cells against inflammation-induced damage.
    Authors: Lian F, Xing X, Yuan G, Schafer C, Rauser S, Walch A, Rocken C, Ebeling M, Wright MB, Schmid RM, Ebert MP, Burgermeister E
    Biochem. J., 2011;438(2):315-23.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Farnesoid X receptor, overexpressed in pancreatic cancer with lymph node metastasis promotes cell migration and invasion.
    Authors: Lee JY, Lee KT, Lee JK, Lee KH, Jang KT, Heo JS, Choi SH, Kim Y, Rhee JC
    Br. J. Cancer, 2011;104(6):1027-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot


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Isotype Controls

Mouse IgG2A Isotype Control

Secondary Antibodies

Reviews for Human FXR/NR1H4 Antibody

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Human FXR/NR1H4 Antibody
By Anonymous on 08/31/2021
Application: WB Sample Tested: Leydig cells Species: Human