Human FXR/NR1H4 Antibody

Catalog # Availability Size / Price Qty
PP-A9033A-00
Product Details
Citations (5)
FAQs
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Human FXR/NR1H4 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human FXR and cross-reacts with rat and mouse FXR. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # A9033A
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human FXR/NR1H4
aa 2-126
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL with the appropriatesecondary reagents to detect human FXR.
 
Immunohistochemistry
This antibody can be used at 20-40 μg/mL with theappropriate secondary reagents to detect human FXR.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Direct ELISA
This antibody can be used at 0.2 μg/mL with the appropriatesecondary reagents to detect human FXR.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: FXR/NR1H4

Farnesoid X-activated Receptor (FXR, HRR-1, BAR, RIP14; NR1H4) is a member of the Orphan Nuclear Receptor family. FXR is expressed in the liver, intestinal villi, renal tubes and adrenal cortex. FXR is a global regulator of bile acid metabolism. Two genes, cholesterol 7a-hydroxylase (CYP7A1) and IBABP (Ileal Bile Acid Binding Protein), which are implicated in bile acid biosynthesis and recycling, respectively, are target genes of FXR. FXR is shown to be transcriptionally activated by farnesol metabolites such as farnesol itself and juvenile hormone III. FXR binds to DNA only as a heterodimer with Retinoid X Receptor (RXR).

Long Name
Farnesoid X-activated receptor
Entrez Gene IDs
9971 (Human); 20186 (Mouse); 60351 (Rat)
Alternate Names
BAR; bile acid receptor; farnesoid X receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; FXR; FXRBAR; HRR-1; HRR1MGC163445; NR1H4; Nuclear receptor subfamily 1 group H member 4; nuclear receptor subfamily 1, group H, member 4; Retinoid X receptor-interacting protein 14; RIP14; RIP14RXR-interacting protein 14

Product Datasheets

Citations for Human FXR/NR1H4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Bile acids down-regulate caveolin-1 in esophageal epithelial cells through sterol responsive element-binding protein.
    Authors: Prade E, Tobiasch M, Hitkova I, Schaffer I, Lian F, Xing X, Tanzer M, Rauser S, Walch A, Feith M, Post S, Rocken C, Schmid RM, Ebert MP, Burgermeister E
    Mol. Endocrinol., 2012;26(5):819-32.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. FXR Controls the Tumor Suppressor NDRG2 and FXR Agonists Reduce Liver Tumor Growth and Metastasis in an Orthotopic Mouse Xenograft Model.
    Authors: Deuschle U, Schuler J, Schulz A, Schluter T, Kinzel O, Abel U, Kremoser C
    PLoS ONE, 2012;7(10):e43044.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  3. Immunomodulatory glycan LNFPIII alleviates hepatosteatosis and insulin resistance through direct and indirect control of metabolic pathways.
    Nat Med, 2012;18(11):1665-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  4. Farnesoid X receptor protects human and murine gastric epithelial cells against inflammation-induced damage.
    Authors: Lian F, Xing X, Yuan G, Schafer C, Rauser S, Walch A, Rocken C, Ebeling M, Wright MB, Schmid RM, Ebert MP, Burgermeister E
    Biochem. J., 2011;438(2):315-23.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  5. Farnesoid X receptor, overexpressed in pancreatic cancer with lymph node metastasis promotes cell migration and invasion.
    Authors: Lee JY, Lee KT, Lee JK, Lee KH, Jang KT, Heo JS, Choi SH, Kim Y, Rhee JC
    Br. J. Cancer, 2011;104(6):1027-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: WB

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

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Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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