Human G-CSF Quantikine QuicKit ELISA Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
39.1 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Natural and recombinant human G-CSF.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine® QuicKit™ Human G-CSF Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human G-CSF levels in cell culture supernates, serum, and plasma. It contains E.
coli-expressed recombinant human G-CSF and antibodies raised against the recombinant protein. Results obtained using natural human G-CSF showed linear curves that were parallel to the standard curves obtained using the QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human G-CSF.
Intra-Assay Precision (Precision within an assay) <div>Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
The recovery of human G-CSF spiked to three levels in samples throughout the range of the assay was evaluated.
||Average % Recovery
|Cell Culture Supernates (n=4)
|EDTA Plasma (n=2)
|Heparin Plasma (n=2)
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human G-CSF were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human G-CSF ELISA Standard Curve
Preparation and Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates
the proliferation, differentiation, and activation of hematopoietic cells in the
neutrophilic granulocyte lineage. Mature human G-CSF is a 178 amino acid (aa) O-glycosylated
protein that contains two intrachain disulfide bridges. In humans, alternate
splicing generates a second minor isoform with a 3 aa deletion. Mouse and human
G-CSF share 76% aa sequence identity, and the two proteins show species
cross-reactivity. G-CSF is produced by activated monocytes and macrophages,
fibroblasts, endothelial cells, astrocytes, neurons, and bone marrow stroma
cells. In addition, various tumor cells express G-CSF constitutively.
receptor (G-CSF R) is a 120 kDa type I transmembrane glycoprotein that belongs to
the hematopoietin receptor superfamily. The mature protein consists of a 603 aa
extracellular domain (ECD), a 23 aa transmembrane segment, and a 186 aa
cytoplasmic domain. The ECD contains an N-terminal Ig-like domain, a cytokine
receptor homology domain, and three fibronectin type III domains. Alternate
splicing of human G-CSF R generates additional isoforms including a potentially
soluble form of the receptor. The ECDs of mouse and human G-CSF R share 63% aa
sequence identity. G-CSF R forms a complex with the ligand in a 2:2 ratio. It
is expressed on monocytes, neutrophils, megakaryocytes, platelets, myeloid
progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell
G-CSF is an
important regulator for granulopoiesis in vivo, and mutations in G-CSF R are associated
with congenital neutropenia. G-CSF can support the growth of multilineage
hematopoietic progenitor cells and mobilize them from the bone marrow into the bloodstream.
G-CSF enhances the functional capacity of mature neutrophils and supports their
survival by limiting the rate of apoptosis. G-CSF also enhances M-CSF induced
monocytopoiesis from hematopoietic progenitor cells and stimulates the proliferation
of peripheral Th2-inducing dendritic cells (30, 31). It promotes the
development of T cell immune tolerance as well as tissue recovery following
myocardial infarction and cerebral ischemia.
Granulocyte Colony Stimulating Factor
Entrez Gene IDs:
1440 (Human); 12985 (Mouse)
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin