Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all GAB2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Gab2
Gln82-Phe292
Accession # Q9UQC2

Specificity

Detects endogenous human Gab2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Gab2 Antibody

Detection of Human Gab2 antibody by Western Blot.

Detection of Human Gab2 by Western Blot.

Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, U937 human histiocytic lymphoma cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human Gab2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4877) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Gab2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Gab2 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: MO7e human megakaryocytic leukemic cell line, U937 human histiocytic lymphoma cell line, and A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GAB2

Gab2 (GRB2-associated binding protein 2) is a 95 kDa member of the Gab family of proteins. It is largely expressed in T and B cells, and serves as an adaptor molecule for the transmission of signals from cytokine and growth factor receptors. Bcr-Abl signals through Gab2, and Gab2 activates ERK. Human Gab2 is 676 amino acids (aa) in length, and contains one pleckstrin homology domain (aa 6‑117) and one methyl binding domain sequence (aa 454‑525). Multiple phosphorylation sites exist, with S159 being an important regulatory site. One alternate start site is reported at M39. Over aa 82‑292, human Gab2 is 93% and 98% aa identical to mouse and canine Gab2, respectively.

Long Name

GRB2-associated Binding Protein 2

Alternate Names

p100

Entrez Gene IDs

9846 (Human); 14389 (Mouse); 84477 (Rat)

Gene Symbol

GAB2

UniProt

Q9UQC2

Additional GAB2 Products

Product Documents for Human Gab2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Gab2 Antibody

For research use only

Related Research Areas

GDNF Family

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail