Human Galectin-1 Quantikine ELISA Kit

R&D Systems | Catalog # DGAL10

R&D Systems
100% Guarantee

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (50 µL), Human Milk (50 µL)

Sensitivity

0.129 ng/mL

Assay Range

0.313-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk)
View all Galectin-1 ELISA Kits »

Product Summary for Human Galectin-1 Quantikine ELISA Kit

The Quantikine Human Galectin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains E. coli-expressed recombinant human Galectin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Galectin-1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Galectin-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 1.96 4.66 8.51 2.07 5.02 9.37
Standard Deviation 0.139 0.408 0.483 0.197 0.444 0.701
CV% 7.1 8.8 5.7 9.5 8.8 7.5

Recovery for Human Galectin-1 Quantikine ELISA Kit

The recovery of Galectin-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 93-115
EDTA Plasma (n=4) 96 87-102
Heparin Plasma (n=4) 93 80-102
Serum (n=4) 96 82-104

Linearity

To assess the linearity of the assay, samples containing high concentrations of Galectin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Galectin-1 ELISA Linearity

Scientific Data Images for Human Galectin-1 Quantikine ELISA Kit

Human Galectin-1 ELISA Standard Curve

Human Galectin-1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-1

Galectin-1, also known as L-14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds Laminin, Fibronectin, Galectin-3BP/MAC-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity.

 

Alternate Names

BHL, Galaptin, Galectin1, GBP, L-14, LGALS1

Entrez Gene IDs

3956 (Human); 16852 (Mouse)

Gene Symbol

LGALS1

Additional Galectin-1 Products

Product Documents for Human Galectin-1 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Galectin-1 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Galectin-1 Quantikine ELISA Kit

Customer Reviews for Human Galectin-1 Quantikine ELISA Kit (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Galectin-1 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Human Galectin-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Blood mononuclear cells (PBMCs)
    Verified Customer | Posted 01/16/2020
    Human Galectin-1 Quantikine ELISA Kit DGAL10
  • Human Galectin-1 Quantikine ELISA Kit
    Name: Mallika Alvala
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 08/27/2018
  • Human Galectin-1 Quantikine ELISA Kit
    Name: Juliana Redondo
    Sample Tested: plasma and cell culture supernate (from mesenchymal stem cells)
    Verified Customer | Posted 11/24/2016
    Plasma 10 fold dilution and cell culture supernate 1 fold dilution
    Human Galectin-1 Quantikine ELISA Kit DGAL10

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

Protocols

View specific protocols for Human Galectin-1 Quantikine ELISA Kit (DGAL10):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits