Human Galectin-1 Quantikine ELISA Kit

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DGAL10
Human Galectin-1 ELISA Standard Curve
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Product Details
Procedure
Citations (14)
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Reviews (3)

Human Galectin-1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL), Human Milk (50 uL)
Sensitivity
0.129 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk)
Specificity
Natural and recombinant human Galectin-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Galectin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains E. coli-expressed recombinant human Galectin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Galectin-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 1.96 4.66 8.51 2.07 5.02 9.37
Standard Deviation 0.139 0.408 0.483 0.197 0.444 0.701
CV% 7.1 8.8 5.7 9.5 8.8 7.5

Recovery

The recovery of Galectin-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 93-115
EDTA Plasma (n=4) 96 87-102
Heparin Plasma (n=4) 93 80-102
Serum (n=4) 96 82-104

Linearity

To assess the linearity of the assay, samples containing high concentrations of Galectin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Galectin-1 ELISA Linearity

Scientific Data

Human Galectin-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-1

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Entrez Gene IDs:
3956 (Human); 16852 (Mouse)
Alternate Names:
14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Galectin-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Bifidobacteria shape antimicrobial T-helper cell responses during infancy and adulthood
    Authors: Vogel, K;Arra, A;Lingel, H;Bretschneider, D;Prätsch, F;Schanze, D;Zenker, M;Balk, S;Bruder, D;Geffers, R;Hachenberg, T;Arens, C;Brunner-Weinzierl, MC;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Increased Angiopoietin-1 and -2 levels in human vitreous are associated with proliferative diabetic retinopathy
    Authors: T Tsai, M Alwees, MA Asaad, J Theile, V Kakkassery, HB Dick, T Schultz, SC Joachim
    PLoS ONE, 2023-01-20;18(1):e0280488.
    Species: Human
    Sample Types: Vitreous Humor
  3. Elevated serum galectin-1 concentrations are associated with increased risks of mortality and acute kidney injury in critically ill patients
    Authors: RH Chou, CT Tsai, YW Lu, JY Guo, CT Lu, YL Tsai, CH Wu, SJ Lin, RY Lien, SF Lu, SF Yang, PH Huang
    PLoS ONE, 2021-09-24;16(9):e0257558.
    Species: Human
    Sample Types: Serum
  4. Validation of galectin-1 as potential diagnostic biomarker of early rheumatoid arthritis
    Authors: A Triguero-M, H de la Fuen, N Montes, AM Ortiz, E Roy-Vallej, S Castañeda, I González-A, A Lamana
    Sci Rep, 2020-10-20;10(1):17799.
    Species: Human
    Sample Types: Serum
  5. Galactin-1, 3 and 9: Potential biomarkers in idiopathic pulmonary fibrosis and other interstitial lung diseases
    Authors: M d'Alessand, E De Vita, L Bergantini, MA Mazzei, S di Valvaso, M Bonizzoli, A Peris, P Sestini, E Bargagli, D Bennett
    Respir Physiol Neurobiol, 2020-09-11;282(0):103546.
    Species: Human
    Sample Types: Serum
  6. Novel benzimidazole-triazole hybrids as apoptosis inducing agents in lung cancer: Design, synthesis, 18F-radiolabeling & galectin-1 inhibition studies
    Authors: N Sridhar Go, V Pooladanda, K Muni Chand, PS Lakshmi So, R Alvala, P Kumar, C Nagaraj, R Dawn Bhara, IA Qureshi, C Godugu, M Alvala
    Bioorg. Chem., 2020-07-22;102(0):104125.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Synthesis, 18F-radiolabeling and apoptosis inducing studies of novel 4, 7-disubstituted coumarins
    Authors: NS Goud, VK Kanth Maka, J Pranay, R Alvala, IA Qureshi, P Kumar, RD Bharath, C Nagaraj, S Yerramsett, M Pal-Bhadra, M Alvala
    Bioorg. Chem., 2020-02-25;97(0):103663.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Increased circulating galectin-1 levels are associated with the progression of kidney function decline in patients undergoing coronary angiography
    Authors: CS Kuo, RH Chou, YW Lu, YL Tsai, PH Huang, SJ Lin
    Sci Rep, 2020-01-29;10(1):1435.
    Species: Human
    Sample Types: Serum
  9. Synthesis of 1-benzyl-1H-benzimidazoles as galectin-1 mediated anticancer agents
    Authors: NS Goud, SM Ghouse, J Vishnu, D Komal, V Talla, R Alvala, J Pranay, J Kumar, IA Qureshi, M Alvala
    Bioorg. Chem., 2019-05-28;89(0):103016.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Elevated Inflammatory Plasma Biomarkers in Patients With Fabry Disease: A Critical Link to Heart Failure With Preserved Ejection Fraction
    Authors: H Yogasundar, A Nikhanj, BN Putko, M Boutin, S Jain-Ghai, A Khan, C Auray-Blai, ML West, GY Oudit
    J Am Heart Assoc, 2018-11-06;7(21):e009098.
    Species: Human
    Sample Types: Plasma
  11. Radiotherapy induces responses of lung cancer to CTLA-4 blockade
    Authors: SC Formenti, NP Rudqvist, E Golden, B Cooper, E Wennerberg, C Lhuillier, C Vanpouille, K Friedman, L Ferrari de, KW Wucherpfen, A Heguy, N Imai, S Gnjatic, RO Emerson, XK Zhou, T Zhang, A Chachoua, S Demaria
    Nat. Med., 2018-11-05;0(0):.
    Species: Human
    Sample Types: Serum
  12. Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
    Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
    Sci Rep, 2017-01-23;7(0):40994.
    Species: Human
    Sample Types: Serum
  13. Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia.
    Authors: Conforti A, Biagini S, Del Bufalo F, Sirleto P, Angioni A, Starc N, Li Pira G, Moretta F, Proia A, Contoli B, Genovese S, Ciardi C, Avanzini M, Rosti V, Lo-Coco F, Locatelli F, Bernardo M
    PLoS ONE, 2013-11-07;8(11):e76989.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Adipose-derived mesenchymal stem cells exert antiinflammatory effects on chondrocytes and synoviocytes from osteoarthritis patients through prostaglandin E2.
    Authors: Manferdini C, Maumus M, Gabusi E, Piacentini A, Filardo G, Peyrafitte J, Jorgensen C, Bourin P, Fleury-Cappellesso S, Facchini A, Noel D, Lisignoli G
    Arthritis Rheum, 2013-05-01;65(5):1271-81.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human Galectin-1 Quantikine ELISA Kit

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Human Galectin-1 Quantikine ELISA Kit
By Anonymous on 01/16/2020
Sample Tested: Blood mononuclear cells (PBMCs)

Human Galectin-1 Quantikine ELISA Kit
By Mallika Alvala on 08/27/2018
Sample Tested: Cell culture supernatant

Human Galectin-1 Quantikine ELISA Kit
By Juliana Redondo on 11/24/2016
Sample Tested: plasma and cell culture supernate (from mesenchymal stem cells)

Plasma 10 fold dilution and cell culture supernate 1 fold dilution