Solid Phase Sandwich ELISA
96-well strip plate
0.313 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk,)
Natural and recombinant human Galectin-1
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Human Galectin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains E. coli-expressed recombinant human Galectin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Galectin-1.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays Three samples of a known concentration were tested in separate assays to
assess inter-assay precision. Assays were performed by at least three
technicians using two lots of kit components.Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk
|Standard Deviation||0.139||0.408||0.483||0.197||0.444||0.701 |
The recovery of Galectin-1 spiked to levels throughout the range of the assay was evaluated.
|Sample Type ||Average % Recovery ||Range % |
|Cell Culture Media (n=4) ||102 ||93-115 |
|EDTA Plasma (n=4) ||96 ||87-102 |
|Heparin Plasma (n=4) ||93 ||80-102 |
|Serum (n=4) ||96 ||82-104 |
To assess the linearity of the assay, samples containing high
concentrations of Galectin-1 were serially diluted with Calibrator Diluent
to produce samples with values within the dynamic range of the assay.
Preparation and Storage
The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.
BHL; Galaptin; GBP; L-14; LGALS1; 14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; DKFZp686E23103; GAL1; gal-1; Gal-1; Galaptin; galaptin; galectin 1; galectin-1; GBP; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; Putative MAPK-activating protein PM12; S-Lac lectin 1;
Related Research Areas
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Showing Results 1 - 1 of 1
- Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia.
Authors: Conforti A, Biagini S, Del Bufalo F, Sirleto P, Angioni A, Starc N, Li Pira G, Moretta F, Proia A, Contoli B, Genovese S, Ciardi C, Avanzini M, Rosti V, Lo-Coco F, Locatelli F, Bernardo M
PLoS ONE, 2013;8(11):e76989.
Sample Type: Cell Culture Supernates
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