Human GFI-1 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF3540R Datasheet / COA / SDS

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AF3540R-100UG

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Human GFI-1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GFI-1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GFI-1
Pro2-Leu250
Accession # Q99684

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GFI-1

Human GFI-1 is a 55 kDa, 422 amino acid (aa) nuclear, zinc-finger transcriptional regulator. It contains an N-terminal SNAG domain, an Ala/Gly-rich region, and a C‑terminus with six C2H2-type zinc finger motifs. GFI-1 binds DNA in a sequence-specific manner. GFI-1 functions as a transcription repressor in lymphoid cells and is required for neutrophil maturation. It also regulates self-renewal and is essential for the functional integrity of hematopoietic stem cells. Over the region used for immunization, human GFI-1 shares less than 50% amino acid sequence homology with human GFI-1B. It also shares 79% and 89% aa sequence identity with mouse and canine GFI-1, respectively.

Long Name

Growth Factor Independent 1

Entrez Gene IDs

2672 (Human); 14581 (Mouse); 24388 (Rat)

Alternate Names

GFI1; GFI-1; growth factor independence-1; growth factor independent 1 transcription repressor; growth factor independent 1; Growth factor independent protein 1; Pal-1; SCN2; Zinc finger protein 163; zinc finger protein Gfi-1; ZNF163; ZNF163FLJ94509