Human GFI-1 Antibody

Catalog #: AF3540
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3540
AF3540-SP

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Detection of GFI-1 in THP-1 Human Cell Line by Flow Cytometry.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GFI-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GFI-1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GFI-1
Pro2-Leu250
Accession # Q99684

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human GFI‑1

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of GFI-1 antibody in THP-1 Human Cell Line antibody by Flow Cytometry.

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Detection of GFI-1 in THP-1 Human Cell Line by Flow Cytometry. THP-1 monocytic leukemia cell line was stained with Goat Anti-Human GFI-1SOX2 Affinity Purified Polyclonal Antibody (Catalog # AF3540, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFI-1

Human GFI-1 is a 55 kDa, 422 amino acid (aa) nuclear, zinc-finger transcriptional regulator. It contains an N-terminal SNAG domain, an Ala/Gly-rich region, and a C‑terminus with six C2H2-type zinc finger motifs. GFI-1 binds DNA in a sequence-specific manner. GFI-1 functions as a transcription repressor in lymphoid cells and is required for neutrophil maturation. It also regulates self-renewal and is essential for the functional integrity of hematopoietic stem cells. Over the region used for immunization, human GFI-1 shares less than 50% amino acid sequence homology with human GFI-1B. It also shares 79% and 89% aa sequence identity with mouse and canine GFI-1, respectively.

Long Name

Growth Factor Independent 1

Entrez Gene IDs

2672 (Human); 14581 (Mouse); 24388 (Rat)

Alternate Names

GFI1; GFI-1; growth factor independence-1; growth factor independent 1 transcription repressor; growth factor independent 1; Growth factor independent protein 1; Pal-1; SCN2; Zinc finger protein 163; zinc finger protein Gfi-1; ZNF163; ZNF163FLJ94509

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human GFI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Statin therapy is associated with lower prevalence of gut microbiota dysbiosis
Authors: S Vieira-Sil, G Falony, E Belda, T Nielsen, J Aron-Wisne, R Chakaroun, SK Forslund, K Assmann, M Valles-Col, TTD Nguyen, S Proost, E Prifti, V Tremaroli, N Pons, E Le Chateli, F Andreelli, JP Bastard, LP Coelho, N Galleron, TH Hansen, JS Hulot, C Lewinter, HK Pedersen, B Quinquis, C Rouault, H Roume, JE Salem, NB Søndertoft, S Touch, ME Dumas, SD Ehrlich, P Galan, JP Gøtze, T Hansen, JJ Holst, L Køber, I Letunic, J Nielsen, JM Oppert, M Stumvoll, H Vestergaar, JD Zucker, P Bork, O Pedersen, F Bäckhed, K Clément, J Raes
Nature, 2020-05-06;581(7808):310-315.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Transcription factor induced conversion of human fibroblasts towards the hair cell lineage
Authors: MB Duran Alon, I Lopez Hern, MA de la Fuen, J Garcia-San, F Giraldez, T Schimmang
PLoS ONE, 2018-07-06;13(7):e0200210.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Single-cell analysis of mixed-lineage states leading to a binary cell fate choice.
Authors: Olsson A, Venkatasubramanian M, Chaudhri V, Aronow B, Salomonis N, Singh H, Grimes H
Nature, 2016-08-31;537(7622):698-702.
Species: Mouse
Sample Types: Protein
Applications: Western Blot
Threshold Levels of Gfi1 Maintain E2A Activity for B Cell Commitment via Repression of Id1
PLoS ONE, 2016-07-28;11(7):e0160344.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot