IFI30 (Gamma-interferon-inducible protein IP-30; also gamma-interferon [IFN-gamma ] inducible lysosomal thiol reductase/GILT and Legumaturain) is a 25-30 kDa member of the GILT family of proteins. It is constitutively expressed in B cells and dendritic cells, and induced by IFN-gamma in non-APCs. IFI30 is both intracellular and secreted as an inactive glycosylated proenzyme. The glycosylation pattern contains a terminal phosphorylated mannose, which is recognized by cell surface mannose-6 phosphate receptors and internalized into lysosomes. In lysosomes, IFI30 is processed into an active, mature form, and via a thiol reductase domain, breaks disulfide bonds in molecules destined for lysosomal degradation. This is a critical first step in the processing and subsequent presentation of peptides that will initiate an antigenic response. The human IFI30 proenzyme is 224 amino acids (aa) in length. In this form, it is 33-35 kDa in size. Following proteolytic processing at both the
N- and C-terminus, a 175 aa, 25-30 kDa active mature form is generated (aa 58-232). The mature region possesses a thiol reductase domain (aa 62-151) plus one utilized Thr phosphorylation site. Both the pro- and mature forms exhibit enzymatic activity. IFI30 is known to exist as a 50-60 kDa disulfide-linked homodimer. There are four potential isoform variants. One contains a 26 aa substitution for aa 213-250, a second shows a deletion of aa 131-161, a third shows a deletion of aa 106-123, while a fourth shows a deletion of aa 64-212. Over aa 27-250, human IFI30 shares 62% aa sequence identity with mouse IFI30.
Human GILT/IFI30 Alexa Fluor® 488‑conjugated Antibody
R&D Systems | Catalog # AF7715G
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ser27-Lys250
Accession # P13284
Specificity
Clonality
Host
Isotype
Applications for Human GILT/IFI30 Alexa Fluor® 488‑conjugated Antibody
Immunohistochemistry
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: GILT/IFI30
Long Name
Alternate Names
Gene Symbol
UniProt
Additional GILT/IFI30 Products
Product Documents for Human GILT/IFI30 Alexa Fluor® 488‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human GILT/IFI30 Alexa Fluor® 488‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Related Research Areas
Customer Reviews for Human GILT/IFI30 Alexa Fluor® 488‑conjugated Antibody
There are currently no reviews for this product. Be the first to review Human GILT/IFI30 Alexa Fluor® 488‑conjugated Antibody and earn rewards!
Have you used Human GILT/IFI30 Alexa Fluor® 488‑conjugated Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars