Human GLP-2R APC-conjugated Antibody

Catalog #: FAB4285A Datasheet / COA / SDS

Discontinued Product

FAB4285A has been discontinued.
View all GLP-2R products.
Detection of GLP‑2R in NS0 Mouse Cell Line Transfected with Human GLP-2R by Flow Cytometry.
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Human GLP-2R APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GLP‑2R. Stains human GLP-2R transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 413801
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human GLP-2R
Met1-Ile553
Accession # O95838
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GLP-2R antibody in NS0 Mouse Cell Line Transfected with Human GLP-2R antibody by Flow Cytometry. View Larger

Detection of GLP‑2R in NS0 Mouse Cell Line Transfected with Human GLP-2R by Flow Cytometry. NS0 mouse myeloma cell line transfected with human GLP-2R was stained with Mouse Anti-Human GLP-2R APC-conjugated Monoclonal Antibody (Catalog # FAB4285A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GLP-2R

GLP-2R is a member of the class II glucagon-secretin G protein-coupled receptor superfamily. It is expressed on enteroendocrine cells in the gut where it mediates the positive effects of GLP2 on villus height, crypt depth and intestinal energy absorbtion. After stimulating a cAMP response, lipid raft-dependent internalization of GLP‑2R results in rapid desensitization to GLP2. Extracellular portions of human GLP-2R show approximately 80% amino acid identity with corresponding regions of mouse GLP-2R.

Long Name
Glucagon-like Peptide 2 Receptor
Entrez Gene IDs
9340 (Human); 93896 (Mouse); 60432 (Rat)
Alternate Names
GLP-2 receptor; GLP2R; GLP-2R; GLP-2-R; glucagon-like peptide 2 receptor

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