Human Glutathione S-Transferase pi 1/GSTP1 Antibody (800027)

R&D Systems | Catalog # MAB6455

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 800027
View all Glutathione S-Transferase pi 1/GSTP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glutathione S-Transferase pi 1/GSTP1
Met1-Glu210
Accession # AAC51280

Specificity

Detects human Glutathione S-Transferase pi 1/GSTP1 in direct ELISAs and Western blots. Detects mouse and rat Glutathione S-Transferase pi 1/GSTP1 in Western Blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Glutathione S-Transferase pi 1/GSTP1 Antibody (800027)

Detection of Human, Mouse, and Rat Glutathione S-Transferase pi 1/GSTP1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Glutathione S-Transferase pi 1/GSTP1 by Western Blot.

Western blot shows lysates of human placenta tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase pi 1/GSTP1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Glutathione S-Transferase pi 1/GSTP1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Glutathione S-Transferase pi 1/GSTP1 in HeLa Human Cell Line.

Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Glutathione S-Transferase pi 1/GSTP1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

Glutathione S-Transferase pi 1/GSTP1 in BG01V Human Embryonic Stem Cells.

Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human and Rat Glutathione S-Transferase pi 1/GSTP1 antibody by Simple WesternTM.

Detection of Human and Rat Glutathione S-Transferase pi 1/GSTP1 by Simple WesternTM.

Simple Western lane view shows lysates of human placenta tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Glutathione S-Transferase pi 1/GSTP1 at approximately 26 kDa (as indicated) using 12.5 µg/mL of Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Glutathione S-Transferase pi 1/GSTP1 Antibody (800027)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and BG01V human embryonic stem cells differentiated into hepatocytes

Simple Western

12.5 µg/mL
Sample: Human placenta tissue and rat brain tissue

Western Blot

0.25 µg/mL
Sample: Human placenta tissue, mouse lung tissue, and rat lung tissue

Reviewed Applications

Read 1 review rated 5 using MAB6455 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glutathione S-Transferase pi 1/GSTP1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTP1 is a cytosolic protein that belongs to pi class of the GST superfamily. It is crystallized as a homodimer (1), but also exists in solution as an equilibrium mixture of monomer and dimer, depending on the protein concentration (2). Four genetic variants of GSTP1 with different enzymatic activities have been identified, which indicates the particular allelic form expressed in tissues could contribute to variation in catalytic efficiency and biological functions (3, 4). Human GSTP1 is present at elevated levels in many tumor cells, and has unique properties as a cancer marker (5). Genetic polymorphisms and expression patterns of GSTP1 have been associated with a variety of effects on human cancer, anti-cancer drug resistance, and asthma (6). In addition to its role as a drug-metabolizing enzyme, GSTP1 has ligand binding properties and regulates kinase signaling pathways through protein-protein interactions (7).

References

  1. Reinemer, P. et al. (1992) J. Mol. Biol. 227:214.
  2. Huang, Y.C. et al. (2008) J. Biol. Chem. 283:32880.
  3. Ali-Osman, F. et al. (1997) J. Biol. Chem. 272:10004.
  4. Hu, X. et al. (1998) Cancer Res. 58:5340.
  5. Sato, K. et al. (1992) Tohoku J. Exp. Med. 168:97.
  6. Townsend, D.M. and K.D. Tew (2003) Oncogene 22:7369.
  7. Adler, V. et al. (1999) EMBO J. 18:1321.

Alternate Names

DFN7, FAEES3, Glutathione STransferase pi 1, GSTP1

Entrez Gene IDs

2950 (Human)

Gene Symbol

GSTP1

UniProt

AAC51280

Additional Glutathione S-Transferase pi 1/GSTP1 Products

Product Documents for Human Glutathione S-Transferase pi 1/GSTP1 Antibody (800027)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Glutathione S-Transferase pi 1/GSTP1 Antibody (800027)

For research use only

Citations for Human Glutathione S-Transferase pi 1/GSTP1 Antibody (800027)

Customer Reviews for Human Glutathione S-Transferase pi 1/GSTP1 Antibody (800027) (1)

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  • Human Glutathione S-Transferase pi 1/GSTP1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HeLa human cervical epithelial carcinoma cell line
    Species: Human
    Verified Customer | Posted 03/22/2022
    Human Glutathione S-Transferase pi 1/GSTP1 Antibody (800027) MAB6455

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