Human Glutathione S-Transferase pi 1/GSTP1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glutathione S-Transferase pi 1/GSTP1 in direct ELISAs and Western blots. Detects mouse and rat Glutathione S-Transferase pi 1/GSTP1 in Western Blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 800027
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Glutathione S-Transferase pi 1/GSTP1
    Met1-Glu210
    Accession # AAC51280
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    12.5 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human, Mouse, and Rat Glutathione
S-Transferase pi 1/GSTP1 by Western Blot.
Western blot shows lysates of human placenta tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Glutathione
S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase pi 1/GSTP1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Glutathione S-Transferase pi 1/GSTP1 in HeLa Human Cell Line. Glutathione
S-Transferase pi 1/GSTP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

Glutathione S-Transferase pi 1/GSTP1 in BG01V Human Embryonic Stem Cells. Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of Human and Rat Glutathione S-Transferase pi 1/GSTP1 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Glutathione
S-Transferase pi 1/GSTP1 at approximately 26 kDa (as indicated) using 12.5 µg/mL of Mouse Anti-Human Glutathione
S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutathione S-Transferase pi 1/GSTP1
Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTP1 is a cytosolic protein that belongs to pi class of the GST superfamily. It is crystallized as a homodimer (1), but also exists in solution as an equilibrium mixture of monomer and dimer, depending on the protein concentration (2). Four genetic variants of GSTP1 with different enzymatic activities have been identified, which indicates the particular allelic form expressed in tissues could contribute to variation in catalytic efficiency and biological functions (3, 4). Human GSTP1 is present at elevated levels in many tumor cells, and has unique properties as a cancer marker (5). Genetic polymorphisms and expression patterns of GSTP1 have been associated with a variety of effects on human cancer, anti-cancer drug resistance, and asthma (6). In addition to its role as a drug-metabolizing enzyme, GSTP1 has ligand binding properties and regulates kinase signaling pathways through protein-protein interactions (7).
  • References:
    1. Reinemer, P. et al. (1992) J. Mol. Biol. 227:214.
    2. Huang, Y.C. et al. (2008) J. Biol. Chem. 283:32880.
    3. Ali-Osman, F. et al. (1997) J. Biol. Chem. 272:10004.
    4. Hu, X. et al. (1998) Cancer Res. 58:5340.
    5. Sato, K. et al. (1992) Tohoku J. Exp. Med. 168:97.
    6. Townsend, D.M. and K.D. Tew (2003) Oncogene 22:7369.
    7. Adler, V. et al. (1999) EMBO J. 18:1321.
  • Entrez Gene IDs:
    2950 (Human)
  • Alternate Names:
    deafness, X-linked 7; DFN7; EC 2.5.1.18; FAEES3; fatty acid ethyl ester synthase III; glutathione S-transferase P; Glutathione STransferase pi 1; Glutathione S-Transferase pi 1; GST class-pi; GST3DFN7; GSTP; GSTP1; GSTP1-1; PI
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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Isotype Controls
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Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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