Human Glutathione S-Transferase pi 1/GSTP1 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human Glutathione S-Transferase pi 1/GSTP1 in direct ELISAs and Western blots. Detects mouse and rat Glutathione S-Transferase pi 1/GSTP1 in Western Blots.
  
• Source
  Monoclonal Mouse IgG_2B Clone # 800027
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  E. coli-derived recombinant human Glutathione S-Transferase pi 1/GSTP1
  Met1-Glu210
  Accession # AAC51280
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.25 µg/mL
  See below
• Simple Western
  12.5 µg/mL
  See below
• Immunocytochemistry
  8-25 µg/mL
  See below

Western Blot click image to view larger	Detection of Human, Mouse, and Rat Glutathione S-Transferase pi 1/GSTP1 by Western Blot. Western blot shows lysates of human placenta tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase pi 1/GSTP1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry click image to view larger	Glutathione S-Transferase pi 1/GSTP1 in HeLa Human Cell Line. Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry click image to view larger	Glutathione S-Transferase pi 1/GSTP1 in BG01V Human Embryonic Stem Cells. Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Simple Western click image to view larger	Detection of Human and Rat Glutathione S-Transferase pi 1/GSTP1 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Glutathione S-Transferase pi 1/GSTP1 at approximately 26 kDa (as indicated) using 12.5 µg/mL of Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reconstitution
  Sterile PBS to a final concentration of 0.5 mg/mL.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Reinemer, P. et al. (1992) J. Mol. Biol. 227:214.
  2. Huang, Y.C. et al. (2008) J. Biol. Chem. 283:32880.
  3. Ali-Osman, F. et al. (1997) J. Biol. Chem. 272:10004.
  4. Hu, X. et al. (1998) Cancer Res. 58:5340.
  5. Sato, K. et al. (1992) Tohoku J. Exp. Med. 168:97.
  6. Townsend, D.M. and K.D. Tew (2003) Oncogene 22:7369.
  7. Adler, V. et al. (1999) EMBO J. 18:1321.

• Entrez Gene IDs:
  2950 (Human)

• Alternate Names:
  deafness, X-linked 7; DFN7; EC 2.5.1.18; FAEES3; fatty acid ethyl ester synthase III; glutathione S-transferase P; Glutathione STransferase pi 1; Glutathione S-Transferase pi 1; GST class-pi; GST3DFN7; GSTP; GSTP1; GSTP1-1; PI

FAQs

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