Human Glutathione S-Transferase pi 1/GSTP1 Antibody

Catalog # Availability Size / Price Qty
MAB6455
MAB6455-SP
Detection of Human, Mouse, and Rat Glutathione S-Transferase pi 1/GSTP1 by Western Blot.
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Human Glutathione S-Transferase pi 1/GSTP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glutathione S-Transferase pi 1/GSTP1 in direct ELISAs and Western blots. Detects mouse and rat Glutathione S-Transferase pi 1/GSTP1 in Western Blots.
Source
Monoclonal Mouse IgG2B Clone # 800027
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Glutathione S-Transferase pi 1/GSTP1
Met1-Glu210
Accession # AAC51280
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
12.5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Glutathione S-Transferase pi 1/GSTP1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Glutathione S-Transferase pi 1/GSTP1 by Western Blot. Western blot shows lysates of human placenta tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase pi 1/GSTP1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Glutathione S-Transferase pi 1/GSTP1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Glutathione S-Transferase pi 1/GSTP1 in HeLa Human Cell Line. Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Glutathione S-Transferase pi 1/GSTP1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Glutathione S-Transferase pi 1/GSTP1 in BG01V Human Embryonic Stem Cells. Glutathione S-Transferase pi 1/GSTP1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Simple Western Detection of Human and Rat Glutathione S-Transferase pi 1/GSTP1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Rat Glutathione S-Transferase pi 1/GSTP1 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Glutathione S-Transferase pi 1/GSTP1 at approximately 26 kDa (as indicated) using 12.5 µg/mL of Mouse Anti-Human Glutathione S-Transferase pi 1/GSTP1 Monoclonal Antibody (Catalog # MAB6455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione S-Transferase pi 1/GSTP1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTP1 is a cytosolic protein that belongs to pi class of the GST superfamily. It is crystallized as a homodimer (1), but also exists in solution as an equilibrium mixture of monomer and dimer, depending on the protein concentration (2). Four genetic variants of GSTP1 with different enzymatic activities have been identified, which indicates the particular allelic form expressed in tissues could contribute to variation in catalytic efficiency and biological functions (3, 4). Human GSTP1 is present at elevated levels in many tumor cells, and has unique properties as a cancer marker (5). Genetic polymorphisms and expression patterns of GSTP1 have been associated with a variety of effects on human cancer, anti-cancer drug resistance, and asthma (6). In addition to its role as a drug-metabolizing enzyme, GSTP1 has ligand binding properties and regulates kinase signaling pathways through protein-protein interactions (7).

References
  1. Reinemer, P. et al. (1992) J. Mol. Biol. 227:214.
  2. Huang, Y.C. et al. (2008) J. Biol. Chem. 283:32880.
  3. Ali-Osman, F. et al. (1997) J. Biol. Chem. 272:10004.
  4. Hu, X. et al. (1998) Cancer Res. 58:5340.
  5. Sato, K. et al. (1992) Tohoku J. Exp. Med. 168:97.
  6. Townsend, D.M. and K.D. Tew (2003) Oncogene 22:7369.
  7. Adler, V. et al. (1999) EMBO J. 18:1321.
Entrez Gene IDs
2950 (Human)
Alternate Names
deafness, X-linked 7; DFN7; EC 2.5.1.18; FAEES3; fatty acid ethyl ester synthase III; glutathione S-transferase P; Glutathione STransferase pi 1; Glutathione S-Transferase pi 1; GST class-pi; GST3DFN7; GSTP; GSTP1; GSTP1-1; PI

Product Datasheets

Citation for Human Glutathione S-Transferase pi 1/GSTP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mining the nucleus accumbens proteome for novel targets of alcohol self-administration in male C57BL/6J mice
    Authors: S Faccidomo, KS Swaim, BL Saunders, TS Santanam, SM Taylor, M Kim, GT Reid, VR Eastman, CW Hodge
    Psychopharmacology (Berl.), 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human Glutathione S-Transferase pi 1/GSTP1 Antibody
By Anonymous on 03/22/2022
Application: ICC/IF Sample Tested: HeLa human cervical epithelial carcinoma cell line Species: Human