Human GM-CSF Quantikine ELISA Kit

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Human GM-CSF ELISA Cell Culture Supernate Standard Curve
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Product Details
Procedure
Citations (57)
FAQs
Supplemental Products
Reviews (4)

Human GM-CSF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity
3 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human GM-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Forty serum and plasma samples from apparently healthy volunteers were evaluated for the presence of human GM-CSF in this assay. All samples measured less than the lowest Human GM-CSF Standard, 7.8 pg/mL. No medical histories were available for the donors used in this study.

Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate and cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed on days 1 and 5 and assayed for levels of human GM-CSF.

ConditionDay 1 (pg/mL)Day 5 (pg/mL)
Unstimulated 3943
Stimulated7131

Product Summary

The Quantikine Human GM-CSF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure GM-CSF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human GM-CSF accurately. Results obtained using natural human GM-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GM-CSF.

Recovery

The recovery of GM-CSF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 97 85-104
Citrate Plasma (n=5) 101 92-106
EDTA Plasma (n=5) 100 94-105
Heparin Plasma (n=5) 98 92-104
Serum (n=5) 98 93-103

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of GM-CSF in various matrices and diluted with the appropriate Calibrator Diluent to produce values within the dynamic range of the assay.
Human GM-CSF ELISA Linearity

Scientific Data

Human GM-CSF ELISA Cell Culture Supernate Standard Curve

Human GM-CSF ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GM-CSF

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.

Long Name:
Granulocyte Macrophage Growth Factor
Entrez Gene IDs:
1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names:
colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  11.   Aspirate and wash 4 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human GM-CSF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

57 Citations: Showing 1 - 10
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  1. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
    Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
    bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Serum granulocyte-macrophage colony-stimulating factor (GM-CSF) is increased in patients with active radiographic axial spondyloarthritis and persists despite anti-TNF treatment
    Authors: C Papagoras, S Tsiami, A Chrysantho, I Mitroulis, X Baraliakos
    Arthritis Research & Therapy, 2022-08-16;24(1):195.
    Species: Human
    Sample Types: Serum
  3. Mesothelin?specific T cell cytotoxicity against triple negative breast cancer is enhanced by 40s ribosomal protein subunit 3?treated self?differentiated dendritic cells
    Authors: N Jirapongwa, S Thongchot, W Chiraphapp, T Chieochans, D Sa-Nguanra, M Warnnissor, P Thuwajit, PT Yenchitsom, C Thuwajit
    Oncology reports, 2022-05-26;48(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Evaluation of the alpha-casein (CSN1S1) locus as a potential target for a site-specific transgene integration
    Authors: AV Smirnov, GV Kontsevaya, TA Shnaider, AM Yunusova, NA Feofanova, LA Gerlinskay, IA Serova, OL Serov, NR Battulin
    Scientific Reports, 2022-05-14;12(1):7983.
    Species: Mouse
    Sample Types: Milk
  5. miR-124-3p relieves allergic rhinitis by inhibiting dipeptidyl peptidase-4
    Authors: S Zhang, D Dong, Y Zhang, J Wang, L Liu, Y Zhao
    International immunopharmacology, 2021-10-26;101(0):108279.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Development of mesothelin-specific CAR NK-92 cells for the treatment of gastric cancer
    Authors: B Cao, M Liu, J Huang, J Zhou, J Li, H Lian, W Huang, Y Guo, S Yang, L Lin, M Cai, C Zhi, J Wu, L Liang, Y Hu, H Hu, J He, B Liang, Q Zhao, K Zhu
    International journal of biological sciences, 2021-09-03;17(14):3850-3861.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Psoralen inhibits the inflammatory response and mucus production in allergic rhinitis by inhibiting the activator protein�1 pathway and the downstream expression of cystatin?SN
    Authors: W Gao, Z Jin, Y Zheng, Y Xu
    Molecular Medicine Reports, 2021-07-19;24(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. miR-31 attenuates murine allergic rhinitis by suppressing interleukin-13-induced nasal epithelial inflammatory responses
    Authors: F Zhou, P Liu, H Lv, Z Gao, W Chang, Y Xu
    Mol Med Rep, 2020-11-12;23(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Inhibition of purinergic P2X receptor 7 (P2X7R) decreases granulocyte-macrophage colony-stimulating factor (GM-CSF) expression in U251 glioblastoma cells
    Authors: M Drill, KL Powell, LK Kan, NC Jones, TJ O'Brien, JA Hamilton, M Monif
    Sci Rep, 2020-09-09;10(1):14844.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Suppressive effects of S100A8 and S100A9 on neutrophil apoptosis by cytokine release of human bronchial epithelial cells in asthma
    Authors: DH Kim, A Gu, JS Lee, EJ Yang, A Kashif, MH Hong, G Kim, BS Park, SJ Lee, IS Kim
    Int J Med Sci, 2020-02-04;17(4):498-509.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Multilevel defects in the hematopoietic niche in essential thrombocythemia
    Authors: T Sun, M Ju, X Dai, H Dong, W Gu, Y Gao, R Fu, X Liu, Y Huang, W Liu, Y Chi, W Wang, H Li, Y Zhou, L Shi, R Yang, L Zhang
    Haematologica, 2019-07-09;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Sanshool improves UVB-induced skin photodamage by targeting JAK2/STAT3-dependent autophagy
    Authors: D Hao, X Wen, L Liu, L Wang, X Zhou, Y Li, X Zeng, G He, X Jiang
    Cell Death Dis, 2019-01-08;10(1):19.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Glucocorticoid insensitivity by staphylococcal enterotoxin B in keratinocytes of allergic dermatitis is associated with impaired nuclear translocation of the Glucocorticoid Receptor ?
    Authors: K Huang, L Ran, W Wang, R Zhou, X Cai, R Li, Y Li, C Zhou, W He, R Wang
    J. Dermatol. Sci., 2018-11-26;92(3):272-280.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Arming oncolytic reovirus with GM-CSF gene to enhance immunity
    Authors: V Kemp, DJM van den Wo, MGM Camps, T van Hall, P Kinderman, N Pronk-van, RC Hoeben
    Cancer Gene Ther., 2018-11-23;0(0):.
  15. Occupational exposure to ultrafine particles in police officers: no evidence for adverse respiratory effects
    Authors: G Jordakieva, I Grabovac, E Valic, KE Schmidt, A Graff, A Schuster, K Hoffmann-S, C Oberhuber, O Scheiner, A Goll, J Godnic-Cva
    J Occup Med Toxicol, 2018-02-03;13(0):5.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  16. Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression
    Authors: F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini
    Cancer Res., 2017-07-28;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Overexpression of GLP-1 receptors suppresses proliferation and cytokine release by airway smooth muscle cells of patients with chronic obstructive pulmonary disease via activation of ABCA1
    Authors: YH Sun, L He, MY Yan, RQ Zhao, B Li, F Wang, Y Yang, HP Yu
    Mol Med Rep, 2017-05-25;0(0):.
  18. Association between Placental Lesions, Cytokines and Angiogenic Factors in Pregnant Women with Preeclampsia
    Authors: Ingrid C Weel
    PLoS ONE, 2016-06-17;11(6):e0157584.
    Species: Human
    Sample Types: Tissue Homogenates
  19. The Novel Receptor C5aR2 Is Required for C5a-Mediated Human Mast Cell Adhesion, Migration, and Proinflammatory Mediator Production.
    Authors: Pundir P, MacDonald C, Kulka M
    J Immunol, 2015-08-17;195(6):2774-87.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli?
    Authors: Spampinato S, Obermeier B, Cotleur A, Love A, Takeshita Y, Sano Y, Kanda T, Ransohoff R
    PLoS ONE, 2015-07-21;10(7):e0133392.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Phase 1b Trial of Biweekly Intravenous Pexa-Vec (JX-594), an Oncolytic and Immunotherapeutic Vaccinia Virus in Colorectal Cancer.
    Authors: Park S, Breitbach C, Lee J, Park J, Lim H, Kang W, Moon A, Mun J, Sommermann E, Maruri Avidal L, Patt R, Pelusio A, Burke J, Hwang T, Kirn D, Park Y
    Mol Ther, 2015-06-15;23(9):1532-40.
    Species: Human
    Sample Types: Plasma
  22. High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts.
    Authors: Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, van der Kuip H, Wernitznig A, Bernthaler A, Dolznig H, Sommergruber W
    PLoS ONE, 2015-04-28;10(4):e0124283.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Autophagy promotes radiation-induced senescence but inhibits bystander effects in human breast cancer cells.
    Authors: Huang, Yao-Huei, Yang, Pei-Ming, Chuah, Qiu-Yu, Lee, Yi-Jang, Hsieh, Yi-Fen, Peng, Chih-Wen, Chiu, Shu-Jun
    Autophagy, 2014-04-30;10(7):1212-28.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Reduced BMPR2 expression induces GM-CSF translation and macrophage recruitment in humans and mice to exacerbate pulmonary hypertension.
    Authors: Sawada H, Saito T, Nickel N, Alastalo T, Glotzbach J, Chan R, Haghighat L, Fuchs G, Januszyk M, Cao A, Lai Y, Perez V, Kim Y, Wang L, Chen P, Spiekerkoetter E, Mitani Y, Gurtner G, Sarnow P, Rabinovitch M
    J Exp Med, 2014-01-20;211(2):263-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. The evolution of cellular deficiency in GATA2 mutation.
    Authors: Dickinson R, Milne P, Jardine L, Zandi S, Swierczek S, McGovern N, Cookson S, Ferozepurwalla Z, Langridge A, Pagan S, Gennery A, Heiskanen-Kosma T, Hamalainen S, Seppanen M, Helbert M, Tholouli E, Gambineri E, Reykdal S, Gottfreethsson M, Thaventhiran J, Morris E, Hirschfield G, Richter A, Jolles S, Bacon C, Hambleton S, Haniffa M, Bryceson Y, Allen C, Prchal J, Dick J, Bigley V, Collin M
    Blood, 2013-12-17;123(6):863-74.
    Species: Human
    Sample Types: Serum
  26. Prevention of F-actin assembly switches the response to SCF from chemotaxis to degranulation in human mast cells.
    Authors: Smrz D, Bandara G, Beaven M, Metcalfe D, Gilfillan A
    Eur J Immunol, 2013-06-04;43(7):1873-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Increased circulation of galectin-3 in cancer induces secretion of metastasis-promoting cytokines from blood vascular endothelium.
    Authors: Chen C, Duckworth C, Zhao Q, Pritchard D, Rhodes J, Yu L
    Clin Cancer Res, 2013-02-11;19(7):1693-704.
    Species: Human
    Sample Types: Whole Cells
  28. Anti-inflammatory effects of methoxyphenolic compounds on human airway cells.
    Authors: Houser KR, Johnson DK, Ishmael FT
    J Inflamm (Lond), 2012-03-13;9(0):6.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype.
    Authors: Freund A, Patil CK, Campisi J
    EMBO J., 2011-03-11;30(8):1536-48.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia.
    Authors: Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ
    Am. J. Pathol., 2010-09-09;177(5):2472-82.
    Species: Human
    Sample Types: Tissue Homogenates
  31. Shaping of iNKT cell repertoire after unrelated cord blood transplantation.
    Authors: Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debre P, Rio B, Vieillard V, Lubrano-Berthelier C, Alatrakchi N
    Clin. Immunol., 2010-02-13;135(3):364-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
    Authors: Calmy A, Gayet-Ageron A, Montecucco F, Nguyen A, Mach F, Burger F, Ubolyam S, Carr A, Ruxungtham K, Hirschel B, Ananworanich J
    AIDS, 2009-05-15;23(8):929-39.
    Species: Human
    Sample Types: Plasma
  33. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009-01-12;206(1):169-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008-12-01;181(11):8053-67.
    Species: Human
    Sample Types: BALF
  35. Assessment of cervicovaginal cytokine levels following exposure to microbicide Nisin gel in rabbits.
    Authors: Aranha CC, Gupta SM, Reddy KV
    Cytokine, 2008-05-29;43(1):63-70.
    Species: Rabbit
    Sample Types: Vaginal Lavage Fluid
  36. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008-05-27;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Systemic responses after bronchial aspirin challenge in sensitive patients with asthma.
    Authors: Makowska JS, Grzegorczyk J, Bienkiewicz B, Wozniak M, Kowalski ML
    J. Allergy Clin. Immunol., 2007-12-20;121(2):348-54.
    Species: Human
    Sample Types: Serum
  38. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006-11-15;119(10):2313-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
    Authors: Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
    Eur. J. Immunol., 2006-07-01;36(7):1975-84.
    Species: Human
    Sample Types: Serum
  40. Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
    Authors: Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
    J. Immunol., 2006-03-15;176(6):3788-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testing.
    Authors: Anderson ST, Williams AJ, Brown JR, Newton SM, Simsova M, Nicol MP, Sebo P, Levin M, Wilkinson RJ, Wilkinson KA
    Am. J. Respir. Crit. Care Med., 2006-02-02;173(9):1038-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
    Authors: Bartemes KR, Cooper KM, Drain KL, Kita H
    J. Allergy Clin. Immunol., 2005-10-01;116(4):827-35.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Modulation of GM-CSF release by enantiomers of beta-agonists in human airway smooth muscle.
    Authors: Ameredes BT, Calhoun WJ
    J. Allergy Clin. Immunol., 2005-07-01;116(1):65-72.
    Species: Human
    Sample Types: Whole Cells
  44. Specific recognition and killing of glioblastoma multiforme by interleukin 13-zetakine redirected cytolytic T cells.
    Authors: Kahlon KS, Brown C, Cooper LJ, Raubitschek A, Forman SJ, Jensen MC
    Cancer Res., 2004-12-15;64(24):9160-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Effect of ozone exposure on airway responses to inhaled allergen in asthmatic subjects.
    Authors: Chen LL, Tager IB, Peden DB, Christian DL, Ferrando RE, Welch BS, Balmes JR
    Chest, 2004-06-01;125(6):2328-35.
    Species: Human
    Sample Types: BALF
  46. Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue.
    Authors: Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH
    Ann. Neurol., 2004-02-01;55(2):221-35.
    Species: Human
    Sample Types: CSF
  47. Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma.
    Authors: Xiao T, Fujita H, Saeki H, Mitsui H, Sugaya M, Tada Y, Kakinuma T, Torii H, Nakamura K, Asahina A, Tamaki K
    Cytokine, 2003-08-01;23(4):126-32.
    Species: Human
    Sample Types: Serum
  48. Spontaneous circulation of myeloid-lymphoid-initiating cells and SCID-repopulating cells in sickle cell crisis.
    Authors: Lamming CE, 175, Augustin L, Blackstad M, Lund TC, Hebbel RP, Verfaillie CM
    2003-03-01;111(6):811-9.
    Species: Human
    Sample Types: Serum
  49. Autocrine type I IFN and contact with endothelium promote the presentation of influenza A virus by monocyte-derived APC.
    Authors: Qu C, Moran TM, Randolph GJ
    J. Immunol., 2003-01-15;170(2):1010-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
    Authors: Hellman C, 107334, Hallden G, Hylander B, Lundahl J
    Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling, 2003-01-01;131(1):75-81.
    Species: Human
    Sample Types: Peritoneal Fluid
  51. Retroviral interleukin 1alpha gene transfer in bone marrow stromal cells in a primate model: induction of myelopoiesis stimulation.
    Authors: de Revel T, Becard N, Sorg T, Rousseau S, Spano JP, Thiebot H, Methali M, Gras G, Le Grand R, Dormont D
    Br. J. Haematol., 2002-09-01;118(3):875-84.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Cell Culture Supernates
  52. Local immunostimulation induced by intravesical administration of autologous interferon-gamma-activated macrophages in patients with superficial bladder cancer.
    Authors: Pages F, Lebel-Binay S, Vieillefond A, Deneux L, Cambillau M, Soubrane O, Debre B, Tardy D, Lemonne JL, Abastado JP, Fridman WH, Thiounn N
    Clin. Exp. Immunol., 2002-02-01;127(2):303-9.
    Species: Human
    Sample Types: Urine
  53. Distinct hematopoietic support by two human stromal cell lines.
    Authors: Loeuillet C, Bernard G, Remy-Martin J, Saas P, Herve P, Douay L, Chalmers D
    Exp. Hematol., 2001-06-01;29(6):736-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin.
    Authors: Szabowski A, Maas-Szabowski N, Andrecht S, Kolbus A, Schorpp-Kistner M, Fusenig NE, Angel P
    Cell, 2000-11-22;103(5):745-55.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
    Authors: Sato E, Koyama S
    Eur. Respir. J., 2000-11-01;16(5):951-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000-07-17;192(2):219-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Serum levels of interleukins, growth factors and angiogenin in patients with endometrial cancer.
    Authors: Chopra V, Dinh TV, Hannigan EV
    J. Cancer Res. Clin. Oncol., 1997-01-01;123(3):167-72.
    Species: Human
    Sample Types: Serum

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Reviews for Human GM-CSF Quantikine ELISA Kit

Average Rating: 5 (Based on 4 Reviews)

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Have you used Human GM-CSF Quantikine ELISA Kit?

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Human GM-CSF Quantikine ELISA Kit
By Anonymous on 04/05/2022
Sample Tested: Serum

Clear instructions and robust method


Human GM-CSF Quantikine ELISA Kit
By Anonymous on 05/11/2018
Sample Tested: PANC-1 human pancreatic carcinoma cell line

Human GM-CSF Quantikine ELISA Kit
By Anonymous on 04/04/2018
Sample Tested: Serum and Plasma

Human GM-CSF Quantikine ELISA Kit
By Mike Ehrhardt on 04/24/2017
Sample Tested: Serum

The Human GM-CSF Quantikine ELISA kit is used for diagnostic use.