Human GPRC5D APC-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Stains human GPRC5D transfectants but not the parental cell line in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 571961
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human GPRC5D
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPRC5D and eGFP was stained with either (A) Mouse Anti-Human GPRC5D APC‑conjugated Monoclonal Antibody (Catalog # FAB6300A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GPRC5D
G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N‑terminal extracellular region. It is expressed in keratinizing epithelial cells, the pancreas, kidney, small intestine, spleen, and testis. Alternate splicing generates two additional isoforms with substitutions or truncations in the cytoplasmic tail. Human GPRC5D shares approximately 80% aa sequence identity with mouse and rat GPRC5D.
  • Long Name:
    G Protein-coupled Receptor, Family D, Group 5, Member D
  • Entrez Gene IDs:
    55507 (Human); 93746 (Mouse); 500349 (Rat)
  • Alternate Names:
    G protein-coupled receptor, family C, group 5, member D; GPRC5D; G-protein coupled receptor family C group 5 member D; MGC129713; MGC129714; orphan G-protein coupled receptor
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FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 6  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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