Human Granzyme B Quantikine ELISA Kit Summary
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Cell Culture Supernates
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human Granzyme B spiked to levels throughout the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||103||93-114|
Human Granzyme B Quantikine ELISA Kit Linearity Competitor Comparison
Human Granzyme B Standard Curve
Preparation and Storage
Background: Granzyme B
Granzyme B is a member of the granzyme family of serine proteases found specifically in granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis utilizing the substrates in this pathway, such as Caspase 3, Caspase 8 and Bid (3, 4). Recent research indicates expanded Granzyme B functionality to include extracellular roles along with its classical pro-apoptotic function. It has been found that Granzyme B is an important mediator of skin injury, repair and inflammation (4) through extracellular substrates including Laminin, VE-Cadherin, Fibronectin and the proteoglycans Aggrecan (3) and Decorin (4).
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