Human Granzyme B Quantikine ELISA Kit

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DGZB00
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Human Granzyme B Quantikine ELISA Kit Linearity Competitor Comparison
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Human Granzyme B Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL)
Sensitivity
4.8 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates)
Specificity
natural and recombinant human Granzyme B.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Granzyme B Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Granzyme B in culture supernates. It contains NS0-expressed recombinant human Granzyme B and antibodies raised against the recombinant protein. Results obtained using natural human Granzyme B showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Granzyme B

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 312 630 1298 311 615 1208
Standard Deviation 8.04 11 28.7 27.5 56.1 77.2
CV% 2.6 1.7 2.2 8.8 9.1 6.4

Recovery

The recovery of human Granzyme B spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 93-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Granzyme B were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Granzyme B Linerarity

Data Examples

Human Granzyme B Quantikine ELISA Kit Linearity Competitor Comparison

Human Granzyme B Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Granzyme B

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Entrez Gene IDs:
3002 (Human); 14939 (Mouse)
Alternate Names:
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT

FAQs

No product specific FAQs exist for this product, however you may

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