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Human Granzyme B Quantikine ELISA Kit

Catalog #: DGZB00 1 Review Datasheet / COA / SDS
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DGZB00
Control Products Available
QC252: Quantikine Immunoassay Control Set 989 for Human Granzyme B
Human Granzyme B Quantikine ELISA Kit Linearity Competitor Comparison
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Granzyme B Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL)
Sensitivity
4.8 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates)
Specificity
natural and recombinant human Granzyme B.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Granzyme B Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Granzyme B in culture supernates. It contains NS0-expressed recombinant human Granzyme B and antibodies raised against the recombinant protein. Results obtained using natural human Granzyme B showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Granzyme B

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 312 630 1298 311 615 1208
Standard Deviation 8.04 11 28.7 27.5 56.1 77.2
CV% 2.6 1.7 2.2 8.8 9.1 6.4

Recovery

The recovery of human Granzyme B spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 93-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Granzyme B were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Granzyme B Linearity

Scientific Data

N/A Human Granzyme B Quantikine ELISA Kit Linearity Competitor Comparison View Larger

Human Granzyme B Quantikine ELISA Kit Linearity Competitor Comparison

N/A Human Granzyme B Standard Curve View Larger

Human Granzyme B Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Granzyme B

Granzyme B is a member of the granzyme family of serine proteases found specifically in granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis utilizing the substrates in this pathway, such as Caspase 3, Caspase 8 and Bid (3, 4). Recent research indicates expanded Granzyme B functionality to include extracellular roles along with its classical pro-apoptotic function. It has been found that Granzyme B is an important mediator of skin injury, repair and inflammation (4) through extracellular substrates including Laminin, VE-Cadherin, Fibronectin and the proteoglycans Aggrecan (3) and Decorin (4). 

As one of the five Granzymes (A, B, H, K and M) identified in the human genome, Granzyme B (32kDa) (5) is the most widely researched in terms of its biological function and its utility in health and disease (4). It is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro-peptide (residues 19-20), and a mature chain (residues 21-247) (6-8). Once inside granules, Granzyme B is fully processed into the mature chain and becomes an active protease when the pro-peptide, Gly-Glu is removed from the N-terminus by cleavage with Cathepsin C (9). The protease activity of Granzyme B is tightly controlled by Serpin B9/ Protease Inhibitor 9 (9). The amino acid sequence of human Granzyme B is 71%, 69%, and 68% identical to its canine, rat, and mouse counterparts, respectively. 
Granzymes have been shown to modulate inflammation, and Granzyme B plasma levels have been found higher with atopic dermatitis and psoriasis when compared to healthy controls. This is in contrast to Granzyme A plasma levels which remain unchanged (10). Serum from patients with Crohn's disease have significantly higher Granzyme B levels than controls (11). 

Entrez Gene IDs:
3002 (Human); 14939 (Mouse)
Alternate Names:
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GranzymeB; GRB; GrzB; GZMB; HLP; SECT

FAQs

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ELISA Controls

Quantikine Immunoassay Control Set 989 for Human Granzyme B

QC252

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Human Granzyme B Quantikine ELISA Kit
By Anonymous on 02/27/2023
Sample Tested: Cell culture supernatant