Human Growth Hormone Biotinylated Antibody

  (1 citations)     
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Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Detects human Growth Hormone in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant rat Growth Hormone is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Growth Hormone
    Phe27-Phe217
    Accession # CAA23779
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Growth Hormone (Catalog # 1067-GH)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunohistochemistry
Growth Hormone in Human Pituitary. Growth Hormone was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human Growth Hormone Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1067) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Growth Hormone

Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin (3) and leptin (4). The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified (5).

Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types (1, 2). GH stimulates the liver and other tissues to produce IGF-I, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-I. GH, directly or indirectly via IGF-I, can act on B cells, T cells, NK cells, macrophages, and neutrophils to exert immunomodulatory activities (6). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake, and protein synthesis (1, 2, 6).

  • References:
    1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
    2. Le Roith, D. et al. (2001) Endocrine Rev., 22:53.
    3. Kojima, K. et al. (1999) Nature, 402:656.
    4. Tannenbaum, G. et al. (1998) Endocrinol. 139:3871.
    5. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  • Entrez Gene IDs:
    2688 (Human)
  • Alternate Names:
    GH1; GHIGHD1B; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; Somatotropin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Troubleshooting PEG-hGH detection supporting pharmacokinetic evaluation in growth hormone deficient patients.
    Authors: Myler HA, McVay S, Kratzsch J
    J Pharmacol Toxicol Methods, 2010;61(2):92-7.
    Species: Human
    Sample Type: Serum
    Application: Electrochemiluminescent Assay

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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Isotype Controls
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Normal Goat IgG Biotinylated Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

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Streptavidin-Alkaline Phosphatase

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NorthernLights Streptavidin NL557

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NorthernLights Streptavidin NL493

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