Human HB-EGF Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF259
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Human HB-EGF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HB-EGF in ELISAs and Western blots. In direct ELISAs, less than 1% cross reactivity with recombinant mouse HB-EGF is observed. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) AR, rhBTC, recombinant mouse Epigen, and rhEpiregulin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HB-EGF (R&D Systems, Catalog # 259-HE)
Asp63-Leu148
Accession # Q99075
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human HB-EGF (Catalog # 259-HE)

Human HB-EGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human HB‑EGF Antibody (Catalog # AF-259-NA)

Standard: Recombinant Human HB-EGF Protein (Catalog # 259-HE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HB-EGF

HB-EGF was originally purified based on its heparin-binding property and mitogenic activity on BALB-3T3 fibroblasts from the conditioned medium of the human U-937 histiocytic lymphoma cell line. The natural protein has an apparent molecular mass of 19 - 23 kDa and exists in multiple forms as a result of heterogenous O‑glycosylation and/or N-terminal truncation. In addition to fibroblasts, HB-EGF is also a potent mitogen for keratinocytes and smooth muscle cells but not for capillary endothelial cells. HB-EGF is produced in monocytes and macrophages. In addition, transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells (SMC), suggesting that HB-EGF may have an important role in the pathogenesis of atherosclerosis.

HB-EGF is a member of the EGF family of mitogens which also include transforming growth factor-alpha (TGF-alpha ), amphiregulin (AR), rat schwanoma-derived growth factor (SDGF), vaccinia growth factor (VGF), and the various ligands for the HER2/erbB2/neu receptor. All these cytokines are derived from transmembrane precursors that contain one or several EGF structural units in their extracellular domain. Many of these transmembrane precursors are biologically active and seem to play a role in juxtacrine stimulation of adjacent cells. The cDNA for HB-EGF encodes a 204 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha, and AR; HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. HB-EGF is reported to be a more potent SMC mitogen than EGF. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF. A diphtheria toxin receptor that mediates the endocytosis of the bound toxin has been cloned and found to be identical to the transmembrane HB-EGF precursor.

Long Name
Heparin Binding EGF-like Growth Factor
Entrez Gene IDs
1839 (Human); 15200 (Mouse)
Alternate Names
diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); Dtr; DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); Dts; DTSF; HBEGF; HB-EGF; Hegfl; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor

Product Datasheets

Citations for Human HB-EGF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Altered lymph node composition in diphtheria toxin receptor-based mouse models to ablate dendritic cells.
    Authors: van Blijswijk J, Schraml B, Rogers N, Whitney P, Zelenay S, Acton S, Reis E Sousa C
    J Immunol, 2015;194(1):307-15.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Ovarian cancer cell heparan sulfate 6-O-sulfotransferases regulate an angiogenic program induced by heparin-binding epidermal growth factor (EGF)-like growth factor/EGF receptor signaling.
    Authors: Cole C, Rushton G, Jayson G, Avizienyte E
    J Biol Chem, 2014;289(15):10488-501.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development (Detection)
  3. Chronic follicular bronchiolitis requires antigen-specific regulatory T cell control to prevent fatal disease progression.
    Authors: Schmitt E, Haribhai D, Jeschke J, Co D, Ziegelbauer J, Yan K, Iwakura Y, Mishra M, Simpson P, Salzman N, Williams C
    J Immunol, 2013;191(11):5460-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  5. Regulatory T cells prevent catastrophic autoimmunity throughout the lifespan of mice.
    Authors: Kim JM, Rasmussen JP, Rudensky AY
    Nat. Immunol., 2007;8(2):191-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
    Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
    Cell, 2006;124(6):1225-39.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Reviews for Human HB-EGF Biotinylated Antibody

Average Rating: 2 (Based on 1 Review)

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Human HB-EGF Biotinylated Antibody
By Anonymous on 12/04/2017
Application: Flow Sample Tested: Mouse splenocytes Species: Mouse

I was unable to get any staining from this antibody, on cells I know express hDTR

Technical Service has followed up with this customer and has received no response. This antibody was used in an application we cannot guarantee.