Human HB-EGF Antibody

Catalog # Availability Size / Price Qty
AF-259-NA
AF-259-SP

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Cell Proliferation Induced by HB‑EGF and Neutralization by Human HB‑EGF Antibody.
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Product Details
Citations (39)
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Human HB-EGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human HB-EGF in ELISAs and Western blots. In direct ELISAs, less than 1% cross reactivity with recombinant mouse HB-EGF is observed. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) Amphiregulin, rhBetacellulin, rhEpiregulin, and recombinant mouse Epigen is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HB-EGF (R&D Systems, Catalog # 259-HE)
Asp63-Leu148
Accession # Q99075
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human HB-EGF (Catalog # 259-HE)
Immunohistochemistry
5-15 µg/mL
See below

Human HB-EGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human HB‑EGF Biotinylated Antibody (Catalog # BAF259)

Standard: Recombinant Human HB-EGF Protein (Catalog # 259-HE)

Neutralization
Measured by its ability to neutralize HB‑EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. Rubin, J.S. et al. (1991) PNAS 88:415. The Neutralization Dose (ND50) is typically 0.1-0.6 µg/mL in the presence of 20 ng/mL Recombinant Human HB‑EGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by HB‑EGF and Neutralization by Human HB‑EGF Antibody. View Larger

Cell Proliferation Induced by HB‑EGF and Neutralization by Human HB‑EGF Antibody. Recombinant Human HB-EGF (Catalog # 259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB-EGF (20 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA). The ND50 is typically 0.1-0.6 µg/mL.

Immunohistochemistry HB-EGF antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

HB-EGF in Human Placenta. HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑259‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry HB-EGF antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

HB-EGF in Human Placenta. HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Mouse HB-EGF by Western Blot View Larger

Detection of Mouse HB-EGF by Western Blot Dynamic expression and processing of Tgf-alpha and Hb-egf during multistep pancreatic neuroendocrine tumorigenesis (PNET). (A) Relative expression of Egf family members in cDNAs prepared from total RNA extracts of successive stages of neoplastic progression (NI = normal islets; HI = hyperplastic islets; AI = angiogenic islets; IT = islet tumors). Levels of mRNAs are expressed as a percentage of the mGus control mRNA. (B) Western blot analysis of the various forms of Tgf-alpha polypeptides in total protein extracts from successive stages of disease progression. (C) Analogous Western blot analysis of the various forms of Hb-egf polypeptides in total protein extracts from the successive disease stages. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20975924), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human HB-EGF by Western Blot View Larger

Detection of Human HB-EGF by Western Blot BHLHE40-knockdown reduced hypoxia-induced expression of a panel of cytokines and growth factors. a Heatmaps of cytokines and growth factors whose hypoxia-induced expression (1% O2 at 6 h or 48 h, fold-change ≥ 1.5 in two independent experiments) was diminished by BHLHE40-knockdown (KD) in LM cells. The gene expression levels were determined using the Illumina Human HT-12 expression BeadChips. Normalized (quantile normalization) hybridization signals were log2 transformed and standardized by genes across experiment conditions to generate the heatmap. b Heatmaps of a subset of genes list in a whose expression was affected by BHLHE40-KD in LM cells exposed to hypoxia combined with low (1 mM) glucose (1%O2/LG, 4 h). The gene expression levels were determined using the Affymetrix Human Gene 1.0 ST array. c Heatmaps of hypoxia-induced genes whose expression was not significantly affected by BHLHE40-KD in LM cells as determined by the Illumina Human HT-12 expression BeadChips. d Expression of luciferase reporters driven by hypoxia-responsive elements of ITGA6 or LDHA was not affected by BHLHE40 knockout (KO) by CRISPR/Cas9 editing in MDA-MB-231 cells, in the absence or presence of exogenous HIF1A. Luciferase activities were normalized to co-transfected CMV-beta -galactosidase and presented as mean ± SD (n = 6). e Expression of genes in control LM empty vector (EV) and LM BHLHE40-KD cells exposed to 1%O2/LG (4 h). mRNA expression levels were determined by qPCR, normalized to RPL13A, and presented as mean ± SD (n = 6). *p <  0.05 (n = 6, 1%O2/LG vs. untreated control), **p <  0.05 (n = 6, KD vs. EV), one-way ANOVA followed by Tukey’s post-hoc tests. f mRNA and protein expression levels of HBEGF and CTGF in primary xenograft tumors, determined by qPCR and immunoblotting, respectively. *p <  0.05 (n = 6, KD vs. EV), Student’s t test. Representative immunoblotting images of three tumors of KD or EV cells are presented Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30285805), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse HB-EGF by Western Blot View Larger

Detection of Mouse HB-EGF by Western Blot Egfr activity contributes to RT2 tumor growth and angiogenic switching. (A) Relative expression quantified by real-time quantitative PCR of Erbb family members in cDNAs derived from total RNA extracts of successive stages of pancreatic neuroendocrine tumorigenesis from RIP1-Tag2 (RT2) transgenic mice (NI = normal islets; HI = hyperplastic islets; AI = angiogenic islets; IT = islet tumors). Levels of mRNAs are expressed as a percentage of the mGus control mRNA. (B) Western blot analysis of protein extracts from successive stages of RT2-derived lesions. (C) Western blot analysis of protein extracts from RT2-derived tumors 4 h after treatment with a vehicle solution or erlotinib (80 mg/kg). (D-F) Comparison of the average tumor burden of RT2 mice treated daily with a control solution (vehicle) or with (D) gefitinib (80 mg/kg), (E) CI-1033 (80 mg/kg), both from 11.5 to 14.5 weeks of age, or with (F) erlotinib (80 mg/kg), from 12 to 16 weeks of age with an additional vehicle-treated time point at 14 weeks. (G) Comparison of the average number of hemorrhagic angiogenic islets per pancreas of RT2 mice treated daily with a vehicle solution or with erlotinib (80 mg/kg) or CI-1033 (80 mg/kg) from 6 to 9 weeks of age. (N = number of animals per treatment group). *P < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20975924), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HB-EGF

HB-EGF was originally purified based on its heparin-binding property and mitogenic activity on BALB-3T3 fibroblasts from the conditioned medium of the human U-937 histiocytic lymphoma cell line. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogenous O-glycosylation and/or N-terminal truncation. In addition to fibroblasts, HB-EGF is also a potent mitogen for keratinocytes and smooth muscle cells but not for capillary endothelial cells. HB-EGF is produced in monocytes and macrophages. In addition, transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells (SMC), suggesting that HB-EGF may have an important role in the pathogenesis of atherosclerosis.

HB-EGF is a member of the EGF family of mitogens which also include transforming growth factor-alpha (TGF-alpha ), amphiregulin (AR), rat schwanoma-derived growth factor (SDGF), vaccinia growth factor (VGF), and the various ligands for the Her2/ErbB2/neu receptor. All these cytokines are derived from transmembrane precursors that contain one or several EGF structural units in their extracellular domain. Many of these transmembrane precursors are biologically active and seem to play a role in juxtacrine stimulation of adjacent cells. The cDNA for HB-EGF encodes a 204 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha, and AR; HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. HB-EGF is reported to be a more potent SMC mitogen than EGF. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF. A diphtheria toxin receptor that mediates the endocytosis of the bound toxin has been cloned and found to be identical to the transmembrane HB-EGF precursor.

Long Name
Heparin Binding EGF-like Growth Factor
Entrez Gene IDs
1839 (Human); 15200 (Mouse)
Alternate Names
diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); Dtr; DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); Dts; DTSF; HBEGF; HB-EGF; Hegfl; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor

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Citations for Human HB-EGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Gintonin-Induced Wound-Healing-Related Responses Involve Epidermal-Growth-Factor-like Effects in Keratinocytes
    Authors: Won, KJ;Lee, R;Choi, SH;Kim, JH;Hwang, SH;Nah, SY;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. A genetic method specifically delineates Th1-type Treg cells and their roles in tumor immunity
    Authors: Okamoto, M;Sasai, M;Kuratani, A;Okuzaki, D;Arai, M;Wing, JB;Sakaguchi, S;Yamamoto, M;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis
    Authors: Q Yu, I Gamayun, P Wartenberg, Q Zhang, S Qiao, S Kusumakshi, S Candlish, V Götz, S Wen, D Das, A Wyatt, V Wahl, F Ectors, K Kattler, D Yildiz, V Prevot, M Schwaninge, G Ternier, P Giacobini, P Ciofi, TD Müller, U Boehm
    Nature Communications, 2023-03-22;14(1):1588.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. A brainstem circuit for nausea suppression
    Authors: C Zhang, LK Vincelette, F Reimann, SD Liberles
    Cell Reports, 2022-06-14;39(11):110953.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Induction of cellular senescence in fibroblasts through beta1-integrin activation by tenascin-C-derived peptide and its protumor effect
    Authors: M Fujita, M Sasada, M Eguchi, T Iyoda, S Okuyama, T Osawa, K Tsuzuranuk, M Sakamoto, Y Hagihara, M Matsumura, S Osada, H Kodama, Y Higami, F Fukai
    American journal of cancer research, 2021-09-15;11(9):4364-4379.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. RAS specific protease induces irreversible growth arrest via p27 in several KRAS mutant colorectal cancer cell lines
    Authors: CK Stubbs, M Biancucci, V Vidimar, KJF Satchell
    Scientific Reports, 2021-09-09;11(1):17925.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Sensory�neuron-derived TAFA4 promotes macrophage tissue repair functions
    Authors: G Hoeffel, G Debroas, A Roger, R Rossignol, J Gouilly, C Laprie, L Chasson, PV Barbon, A Balsamo, A Reynders, A Moqrich, S Ugolini
    Nature, 2021-05-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Structural basis of malaria RIFIN binding by LILRB1-containing antibodies
    Authors: Y Chen, K Xu, L Piccoli, M Foglierini, J Tan, W Jin, J Gorman, Y Tsybovsky, B Zhang, B Traore, C Silacci-Fr, C Daubenberg, PD Crompton, R Geiger, F Sallusto, PD Kwong, A Lanzavecch
    Nature, 2021-03-31;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  9. Morphological remodeling during recovery of the neuromuscular junction from terminal Schwann cell ablation in adult mice
    Authors: RL Hastings, M Mikesh, YI Lee, WJ Thompson
    Sci Rep, 2020-07-07;10(1):11132.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB?EGF
    Authors: E Fu, T Liu, S Yu, X Chen, L Song, H Lou, F Ma, S Zhang, S Hussain, J Guo, J Sun, P Yu, X Mao, L Wei
    Oncol. Rep., 2020-05-29;44(2):698-710.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. HB-EGF-EGFR Signaling in Bone Marrow Endothelial Cells Mediates Angiogenesis Associated with Multiple Myeloma
    Authors: L Rao, D Giannico, P Leone, AG Solimando, E Maiorano, C Caporusso, L Duda, R Tamma, R Mallamaci, N Susca, A Buonavogli, MC Da Vià, D Ribatti, V De Re, A Vacca, V Racanelli
    Cancers (Basel), 2020-01-10;12(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways
    Authors: NM Miranda da, CT Saab Abe, GP Mitre, RA Mesquita, MSD Silva Kata, AL Ribeiro Ri, RG Jaeger, SM Alves-Júni, AM Smith, JJV Pinheiro
    Cells, 2019-07-17;8(7):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells
    Authors: S Grainger, N Nguyen, J Richter, J Setayesh, B Lonquich, CH Oon, JM Wozniak, R Barahona, CN Kamei, J Houston, M Carrillo-T, IA Drummond, D Gonzalez, K Willert, D Traver
    Nat. Cell Biol., 2019-05-20;21(6):721-730.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. HIF-1&alpha, NOTCH1, ADAM12, and HB-EGF are overexpressed in mucoepidermoid carcinoma
    Authors: DC Branco, NMM da Costa, CTS Abe, MSDS Kataoka, JJV Pinheiro, SM Alves Júni
    Oral Surg Oral Med Oral Pathol Oral Radiol, 2018-10-05;127(1):e8-e17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Vascular Endothelial Growth Factor-A165b Restores Normal Glomerular Water Permeability in a Diphtheria-Toxin Mouse Model of Glomerular Injury
    Authors: M Stevens, CR Neal, AHJ Salmon, DO Bates, SJ Harper, S Oltean
    Nephron, 2018-01-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. M2 macrophages induce ovarian cancer cell proliferation via a heparin binding epidermal growth factor/matrix metalloproteinase 9 intercellular feedback loop
    Authors: MJ Carroll, A Kapur, M Felder, MS Patankar, PK Kreeger
    Oncotarget, 2016-12-27;7(52):86608-86620.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells.
    Authors: Veltmann M, Hollborn M, Reichenbach A, Wiedemann P, Kohen L, Bringmann A
    PLoS ONE, 2016-01-22;11(1):e0147312.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. MicroRNA-132 enhances transition from inflammation to proliferation during wound healing.
    Authors: Li D, Wang A, Liu X, Meisgen F, Grunler J, Botusan I, Narayanan S, Erikci E, Li X, Blomqvist L, DU L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina S, Stahle M, Landen N
    J Clin Invest, 2015-06-29;125(8):3008-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow.
    Authors: Yu V, Saez B, Cook C, Lotinun S, Pardo-Saganta A, Wang Y, Lymperi S, Ferraro F, Raaijmakers M, Wu J, Zhou L, Rajagopal J, Kronenberg H, Baron R, Scadden D
    J Exp Med, 2015-04-27;212(5):759-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. A mouse model of otitis media identifies HB-EGF as a mediator of inflammation-induced mucosal proliferation.
    Authors: Suzukawa, Keigo, Tomlin, Julia, Pak, Kwang, Chavez, Eduardo, Kurabi, Arwa, Baird, Andrew, Wasserman, Stephen, Ryan, Allen F
    PLoS ONE, 2014-07-17;9(7):e102739.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013-12-31;8(12):e85147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. An EGFR wild type-EGFRvIII-HB-EGF feed-forward loop regulates the activation of EGFRvIII.
    Authors: Li L, Chakraborty S, Yang C, Hatanpaa K, Cipher D, Puliyappadamba V, Rehman A, Jiwani A, Mickey B, Madden C, Raisanen J, Burma S, Saha D, Wang Z, Pingle S, Kesari S, Boothman D, Habib A
    Oncogene, 2013-09-30;33(33):4253-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.
    Authors: Diaz B, Yuen A, Iizuka S, Higashiyama S, Courtneidge S
    J Cell Biol, 2013-04-15;201(2):279-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Immature doublecortin-positive hippocampal neurons are important for learning but not for remembering.
    Authors: Vukovic, Jana, Borlikova, Gilyana, Ruitenberg, Marc J, Robinson, Gregory, Sullivan, Robert K, Walker, Tara L, Bartlett, Perry F
    J Neurosci, 2013-04-10;33(15):6603-13.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. Heparin-binding epidermal growth factor-like growth factor eliminates constraints on activated Kras to promote rapid onset of pancreatic neoplasia.
    Authors: Ray, K C, Moss, M E, Franklin, J L, Weaver, C J, Higginbotham, J, Song, Y, Revetta, F L, Blaine, S A, Bridges, L R, Guess, K E, Coffey, R J, Crawford, H C, Washington, M K, Means, A L
    Oncogene, 2013-02-04;33(7):823-31.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  26. Heparin binding epidermal growth factor in renal ischaemia/reperfusion injury.
    Authors: Mulder GM, Nijboer WN, Seelen MA, Sandovici M, Bos EM, Melenhorst WB, Trzpis M, Kloosterhuis NJ, Visser L, Henning RH, Leuvenink HG, Ploeg RJ, Sunnarborg SW, van Goor H
    J. Pathol., 2010-06-01;221(2):183-92.
    Species: Human, Mouse, Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth.
    Authors: Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT
    J. Invest. Dermatol., 2010-04-29;130(8):2031-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors.
    Authors: Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II
    Clin. Cancer Res., 2009-07-21;15(15):4829-37.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR.
    Authors: Monslow J, Sato N, Mack JA, Maytin EV
    J. Invest. Dermatol., 2009-02-19;129(8):2046-58.
    Species: Human, Rat
    Sample Types: Cell Lysates, Recombinant Protein
    Applications: Western Blot
  30. Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components.
    Authors: Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A
    Eur. Respir. J., 2007-09-05;30(6):1064-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
    Authors: Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM
    Am. J. Respir. Cell Mol. Biol., 2007-06-28;37(5):578-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  32. Ras activation in response to phorbol ester proceeds independently of the EGFR via an unconventional nucleotide-exchange factor system in COS-7 cells.
    Authors: Rubio I, Rennert K, Wittig U, Beer K, Durst M, Stang SL, Stone J, Wetzker R
    Biochem. J., 2006-09-01;398(2):243-56.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Role of epidermal growth factor receptor transactivation in alpha1B-adrenoceptor phosphorylation.
    Authors: Casas-Gonzalez P, Garcia-Sainz JA
    Eur. J. Pharmacol., 2006-05-27;542(1):31-6.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  34. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006-05-11;126(9):2096-105.
    Species: Human
    Sample Types: Serum
    Applications: Neutralization
  35. Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
    Authors: Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE
    Development, 2006-01-11;133(4):751-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  36. Expression of heparin-binding epidermal growth factor-like growth factor and its receptors in the human fallopian tube and endometrium after treatment with mifepristone.
    Authors: Sun XX, Gemzell-Danielsson K, Li HZ, Stabi B, Stavreus-Evers A
    Fertil. Steril., 2006-01-01;85(1):171-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  37. Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress.
    Authors: Kim J, Adam RM, Freeman MR
    Cancer Res., 2005-09-15;65(18):8242-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
    Authors: Kansra S, Johnson JL
    Mol. Biol. Cell, 2004-07-14;15(9):4299-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
    Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
    Dev. Biol., 2004-02-15;266(2):223-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization

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Human HB-EGF Antibody
By Tamar Licht on 11/30/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult brain Species: Mouse- transgenic inducible DTR

Perfect to detect expression of transgenic human diphtheria toxin receptor in mouse.
Here I show POMC-Cre line crossed to iDTR line. Mouse hypothalamus is presented. HB-EFG in green.