Human HepaCAM Antibody

Catalog #: MAB4108
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4108
MAB4108-SP

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Detection of Human HepaCAM by Western Blot.

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HepaCAM Antibody Summary

Species Reactivity

Human

Specificity

Detects human HepaCAM in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 419305

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HepaCAM
Val34-Tyr242
Accession # Q14CZ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HepaCAM by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human HepaCAM Monoclonal Antibody (Catalog # MAB4108) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HepaCAM at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HepaCAM

HepaCAM (hepatocyte cell adhesion molecule) is a 50-75 kDa, type I transmembrane glycoprotein that belongs to the Ig-superfamily. It forms homodimers on the cell surface and promotes cell spreading and motility. Human HepaCAM is 382 aa in length. It contains a 206 aa extracellular domain (ECD) (aa 35‑240) and a 152 aa cytoplasmic region. The ECD has two C2 Ig-like domains. There is one potential truncated isoform that shows an alternate start site at Met200. Over aa 34‑242, human HepaCAM shares 99% and 98% aa sequence identity with mouse and dog HepaCAM, respectively.

Long Name

Hepatocyte Cell Adhesion Molecule

Entrez Gene IDs

220296 (Human)

Alternate Names

cancer susceptibility; FLJ25530; glial cell adhesion molecule; GlialCAM; HepaCAM; hepatic and glial cell adhesion molecule; hepatocyte cell adhesion molecule; Protein hepaCAM

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human HepaCAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Neurotoxic reactive astrocytes induce cell death via saturated lipids
Authors: KA Guttenplan, MK Weigel, P Prakash, PR Wijewardha, P Hasel, U Rufen-Blan, AE Münch, JA Blum, J Fine, MC Neal, KD Bruce, AD Gitler, G Chopra, SA Liddelow, BA Barres
Nature, 2021;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Enrichment of Glial Cells From Human Post-mortem Tissue for Transcriptome and Proteome Analysis Using Immunopanning
Authors: A Nolle, I van Dijken, CM Waelti, D Calini, J Bryois, E Lezan, S Golling, A Augustin, L Foo, JJM Hoozemans
Frontiers in Cellular Neuroscience, 2021;15(0):772011.
Species: Human
Sample Types: Whole Cells
Applications: Immunopanning
Knockout of reactive astrocyte activating factors slows disease progression in an ALS mouse model
Authors: KA Guttenplan, MK Weigel, DI Adler, J Couthouis, SA Liddelow, AD Gitler, BA Barres
Nat Commun, 2020;11(1):3753.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture
Tumor-associated reactive astrocytes aid the evolution of immunosuppressive environment in glioblastoma
Authors: D Henrik Hei, VM Ravi, SP Behringer, JH Frenking, J Wurm, K Joseph, NWC Garrelfs, J Strähle, S Heynckes, J Grauvogel, P Franco, I Mader, M Schneider, AL Potthoff, D Delev, UG Hofmann, C Fung, J Beck, R Sankowski, M Prinz, O Schnell
Nat Commun, 2019;10(1):2541.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
HepaCAM associates with connexin 43 and enhances its localization in cellular junctions
Sci Rep, 2016;6(0):36218.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot