Human HepaCAM Antibody

Catalog # Availability Size / Price Qty
MAB4108
MAB4108-SP
Detection of Human HepaCAM by Western Blot.
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Product Details
Citations (5)
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Human HepaCAM Antibody Summary

Species Reactivity
Human
Specificity
Detects human HepaCAM in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 419305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HepaCAM
Val34-Tyr242
Accession # Q14CZ8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human HepaCAM antibody by Western Blot. View Larger

Detection of Human HepaCAM by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human HepaCAM Monoclonal Antibody (Catalog # MAB4108) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HepaCAM at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HepaCAM

HepaCAM (hepatocyte cell adhesion molecule) is a 50-75 kDa, type I transmembrane glycoprotein that belongs to the Ig-superfamily. It forms homodimers on the cell surface and promotes cell spreading and motility. Human HepaCAM is 382 aa in length. It contains a 206 aa extracellular domain (ECD) (aa 35‑240) and a 152 aa cytoplasmic region. The ECD has two C2 Ig-like domains. There is one potential truncated isoform that shows an alternate start site at Met200. Over aa 34‑242, human HepaCAM shares 99% and 98% aa sequence identity with mouse and dog HepaCAM, respectively.

Long Name
Hepatocyte Cell Adhesion Molecule
Entrez Gene IDs
220296 (Human)
Alternate Names
cancer susceptibility; FLJ25530; glial cell adhesion molecule; GlialCAM; HepaCAM; hepatic and glial cell adhesion molecule; hepatocyte cell adhesion molecule; Protein hepaCAM

Product Datasheets

Citations for Human HepaCAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Neurotoxic reactive astrocytes induce cell death via saturated lipids
    Authors: KA Guttenplan, MK Weigel, P Prakash, PR Wijewardha, P Hasel, U Rufen-Blan, AE Münch, JA Blum, J Fine, MC Neal, KD Bruce, AD Gitler, G Chopra, SA Liddelow, BA Barres
    Nature, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Enrichment of Glial Cells From Human Post-mortem Tissue for Transcriptome and Proteome Analysis Using Immunopanning
    Authors: A Nolle, I van Dijken, CM Waelti, D Calini, J Bryois, E Lezan, S Golling, A Augustin, L Foo, JJM Hoozemans
    Frontiers in Cellular Neuroscience, 2021;15(0):772011.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunopanning
  3. Knockout of reactive astrocyte activating factors slows disease progression in an ALS mouse model
    Authors: KA Guttenplan, MK Weigel, DI Adler, J Couthouis, SA Liddelow, AD Gitler, BA Barres
    Nat Commun, 2020;11(1):3753.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Tumor-associated reactive astrocytes aid the evolution of immunosuppressive environment in glioblastoma
    Authors: D Henrik Hei, VM Ravi, SP Behringer, JH Frenking, J Wurm, K Joseph, NWC Garrelfs, J Strähle, S Heynckes, J Grauvogel, P Franco, I Mader, M Schneider, AL Potthoff, D Delev, UG Hofmann, C Fung, J Beck, R Sankowski, M Prinz, O Schnell
    Nat Commun, 2019;10(1):2541.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. HepaCAM associates with connexin 43 and enhances its localization in cellular junctions
    Sci Rep, 2016;6(0):36218.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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