Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) HS3ST4, rhHS2ST1, rhHS6ST1, and recombinant mouse HS6ST3 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3
    Gly60-Asp390
    Accession # Q9Y662
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 by Western Blot. Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line, JAR human choriocarcinoma cell line, and Capan‑1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7276) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3
Heparan sulfate is a highly sulfated polysaccharide found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, is considered to be a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2‑O position by heparan sulfate 2‑O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3‑O, and 6‑O positions by N‑deacetylase/ N‑sulfotransferases (NDSTs), heparan sulfate 3‑O sulfotransferases (HS3STs) and heparan sulfate 6‑O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST3 has two forms, HS3ST3A1 and HS3ST3B1, differing only at the N‑terminus. The two HS3STs have the same substrate specificity (5) and similar tissue distribution with a high levels of expression in the liver and spleen (3, 6). HS3ST3 can sulfate IdoUA2S‑GlcNS, IdoUA2S‑GlcNH2 and IdoUA2S‑GlcNS6S and generate tetrasulfated disaccharide units (3, 6). HS3ST3 is involved in generation of a binding receptor to the herpes simplex virus‑1 (HSV‑1) (7). The enzyme activity was determined using a phosphatase-coupled method (8).
  • References:
    1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
    2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
    3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
    4. Xu, D. et al. (2005) Biochem. J. 386:451.
    5. Liu, J. et al. (1999) J. Biol. Chem. 274:5185.
    6. Mochiziki, H. (2008) J. Biol. Chem. 283:31237.
    7. Moon, A.F. et al. (2004) J. Biol. Chem. 279:45185.
    8. Prather, B. et al. (2012) Anal. Biochem. in press.
  • Long Name:
    Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3B1
  • Entrez Gene IDs:
    9953 (Human); 54710 (Mouse); 303218 (Rat)
  • Alternate Names:
    3OST3B1; h3-OST-3B; heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1; Heparan Sulfate Glucosamine 3OSulfotransferase 3; HS3ST3B1
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