Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antibody

Catalog # Availability Size / Price Qty
AF7276
AF7276-SP
Detection of Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 by Western Blot.
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Citations (2)
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Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) HS3ST4, rhHS2ST1, rhHS6ST1, and recombinant mouse HS6ST3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3
Gly60-Asp390
Accession # Q9Y662
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 by Western Blot. View Larger

Detection of Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 by Western Blot. Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line, JAR human choriocarcinoma cell line, and Capan‑1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7276) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3

Heparan sulfate is a highly sulfated polysaccharide found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, is considered to be a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2‑O position by heparan sulfate 2‑O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3‑O, and 6‑O positions by N‑deacetylase/ N‑sulfotransferases (NDSTs), heparan sulfate 3‑O sulfotransferases (HS3STs) and heparan sulfate 6‑O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST3 has two forms, HS3ST3A1 and HS3ST3B1, differing only at the N‑terminus. The two HS3STs have the same substrate specificity (5) and similar tissue distribution with a high levels of expression in the liver and spleen (3, 6). HS3ST3 can sulfate IdoUA2S‑GlcNS, IdoUA2S‑GlcNH2 and IdoUA2S‑GlcNS6S and generate tetrasulfated disaccharide units (3, 6). HS3ST3 is involved in generation of a binding receptor to the herpes simplex virus‑1 (HSV‑1) (7). The enzyme activity was determined using a phosphatase-coupled method (8).

References
  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
  4. Xu, D. et al. (2005) Biochem. J. 386:451.
  5. Liu, J. et al. (1999) J. Biol. Chem. 274:5185.
  6. Mochiziki, H. (2008) J. Biol. Chem. 283:31237.
  7. Moon, A.F. et al. (2004) J. Biol. Chem. 279:45185.
  8. Prather, B. et al. (2012) Anal. Biochem. in press.
Long Name
Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3B1
Entrez Gene IDs
9953 (Human); 54710 (Mouse); 303218 (Rat)
Alternate Names
3OST3B1; h3-OST-3B; heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1; Heparan Sulfate Glucosamine 3OSulfotransferase 3; HS3ST3B1

Product Datasheets

Citations for Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The heparan sulfate 3-O-sulfotransferases (HS3ST) 2, 3B and 4 enhance proliferation and survival in breast cancer MDA-MB-231 cells
    Authors: C Hellec, M Delos, M Carpentier, A Denys, F Allain
    PLoS ONE, 2018;13(3):e0194676.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. The Pro-Tumoral Activity of Heparan Sulfate 3-O-Sulfotransferase 3B (HS3ST3B) in Breast Cancer MDA-MB-231 Cells Is Dependent on the Expression of Neuropilin-1
    Authors: C Hellec, M Diawara, M Carpentier, A Denys, F Allain
    Molecules, 2018;23(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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