Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antibody
Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antibody Summary
Gly60-Asp390
Accession # Q9Y662
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line, JAR human choriocarcinoma cell line, and Capan-1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7276) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3
Heparan sulfate is a highly sulfated polysaccharide found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, is considered to be a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2‑O position by heparan sulfate 2‑O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3‑O, and 6‑O positions by N‑deacetylase/ N‑sulfotransferases (NDSTs), heparan sulfate 3‑O sulfotransferases (HS3STs) and heparan sulfate 6‑O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST3 has two forms, HS3ST3A1 and HS3ST3B1, differing only at the N‑terminus. The two HS3STs have the same substrate specificity (5) and similar tissue distribution with a high levels of expression in the liver and spleen (3, 6). HS3ST3 can sulfate IdoUA2S‑GlcNS, IdoUA2S‑GlcNH2 and IdoUA2S‑GlcNS6S and generate tetrasulfated disaccharide units (3, 6). HS3ST3 is involved in generation of a binding receptor to the herpes simplex virus‑1 (HSV‑1) (7). The enzyme activity was determined using a phosphatase-coupled method (8).
- Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
- Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
- Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
- Xu, D. et al. (2005) Biochem. J. 386:451.
- Liu, J. et al. (1999) J. Biol. Chem. 274:5185.
- Mochiziki, H. (2008) J. Biol. Chem. 283:31237.
- Moon, A.F. et al. (2004) J. Biol. Chem. 279:45185.
- Prather, B. et al. (2012) Anal. Biochem. in press.
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Citations for Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The heparan sulfate 3-O-sulfotransferases (HS3ST) 2, 3B and 4 enhance proliferation and survival in breast cancer MDA-MB-231 cells
Authors: C Hellec, M Delos, M Carpentier, A Denys, F Allain
PLoS ONE, 2018;13(3):e0194676.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The Pro-Tumoral Activity of Heparan Sulfate 3-O-Sulfotransferase 3B (HS3ST3B) in Breast Cancer MDA-MB-231 Cells Is Dependent on the Expression of Neuropilin-1
Authors: C Hellec, M Diawara, M Carpentier, A Denys, F Allain
Molecules, 2018;23(10):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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