Human Hepassocin/FGL1 Antibody

Catalog #: MAB112302 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB112302-100
MAB112302-SP

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Detection of Hepassocin/FGL1 in Human Liver.
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Human Hepassocin/FGL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Hepassocin/FGL1 in direct ELISA.
Source
Monoclonal Mouse IgG1 Clone # 1052936
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Scientific Data

Immunohistochemistry View Larger

Detection of Hepassocin/FGL1 in Human Liver. Hepassocin/FGL1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB112302) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry View Larger

Detection of Hepassocin/FGL1 in HepG2 Human hepatocellular carcinoma cell line (positive) and K562 Human chronic myelogenous leukemia cell line (negative). Hepassocin/FGL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative) using Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB112302) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and FGL1, is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. Hepassocin may play a role in the development of hepatocellular carcinomas.

Long Name
Fibrinogen Like 1
Entrez Gene IDs
2267 (Human); 246186 (Rat); 102118020 (Cynomolgus Monkey)
Alternate Names
FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1

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