Human Hepsin Antibody

Catalog # Availability Size / Price Qty
MAB4776
MAB4776-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human Hepsin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Hepsin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 506220
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepsin
Ile163-Leu417
Accession # P05981
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Hepsin (Catalog # 4776‑SE) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Hepsin (Catalog # 4776‑SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepsin

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6). rhHepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the recombinant human Hepsin.

References
  1. Leytus, S.P. et al. (1988) Biochemistry 27:1067.
  2. Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
  3. Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
  4. Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
  5. Dhanasekaran, S.M. et al. (2001) Nature 412:822.
  6. Wu, Q. and Parry, G. (2007) Front. Biosci. 12:5052.
Entrez Gene IDs
3249 (Human); 15451 (Mouse); 29135 (Rat)
Alternate Names
EC 3.4.21; EC 3.4.21.106; Hepsin; HPN; serine protease hepsin catalytic chain; serine protease hepsin; TMPRSS1 ; TMPRSS1serine protease hepsin non-catalytic chain; Transmembrane protease serine 1; transmembrane protease, serine 1

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Citation for Human Hepsin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Deregulated hepsin protease activity confers oncogenicity by concomitantly augmenting HGF/MET signalling and disrupting epithelial cohesion.
    Authors: Tervonen T, Belitskin D, Pant S, Englund J, Marques E, Ala-Hongisto H, Nevalaita L, Sihto H, Heikkila P, Leidenius M, Hewitson K, Ramachandra M, Moilanen A, Joensuu H, Kovanen P, Poso A, Klefstrom J
    Oncogene, 2015-07-13;0(0):ePub.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Reviews for Human Hepsin Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human Hepsin Antibody
By Anonymous on 05/22/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

AF4776 was used as the detection antibody in a sandwich ELISA with MAB4776 as the capture antibody.


Human Hepsin Antibody
By Anonymous on 04/20/2019
Application: Flow Sample Tested: MCF 10A human breast epithelial cell line Species: Human

MCF10A cells transduced with an andicible (Doxycyclin) hepsin overexpression construct were induced with 100ng/ml for 48h, trypsinized, blocked with 1%BSA-PBS and stained 1:20 dilution. Secondary antibody Was Alexa488 conjugated. Analysis on BD Accuri.