hh Human HGF R/c‑MET Biotinylated Antibody BAF358: R&D Systems

Human HGF R/c‑MET Biotinylated Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human HGF R/c-MET in ELISAs and Western blots. In Western blot, this antibody shows approximately 50% cross-reactivity with recombinant mouse HGF R.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
    Glu25-Thr932
    Accession # P08581
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human HGF R/c-MET Fc Chimera (Catalog # 358-MT)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
    • Human HGF R/c-MET Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human HGF R/c‑MET Antibody (Catalog # MAB3581)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human HGF R/c‑MET Biotinylated Antibody (Catalog # BAF358)
  • ELISA Standard
     
    Recombinant Human HGF R/c-MET Fc Chimera His-tag Protein (Catalog # 358-MT)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Human HGF R/c‑MET Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF358, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF R/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% aa sequence identity with canine, mouse, and rat HGF R.

  • References:
    1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
    2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
    3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
    4. Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
    5. Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
    6. Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
    7. Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
    8. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
    9. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
    10. Ponzetto, C. et al. (1994) Cell 77:261.
    11. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
    12. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
    13. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
    14. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
    15. Wang, X. et al. (2002) Mol. Cell 9:411.
    16. Trusolino, L. et al. (2001) Cell 107:643.
    17. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
    18. Conrotto, P. et al. (2004) Oncogene 23:5131.
    19. Follenzi, A. et al. (2000) Oncogene 19:3041.
    20. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
  • Long Name:
    Hepatocyte Growth Factor Receptor
  • Entrez Gene IDs:
    4233 (Human); 17295 (Mouse)
  • Alternate Names:
    AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Tumor and Plasma Met Levels in Non-Metastatic Prostate Cancer
    Authors: Deborah R Kaye
    PLoS ONE, 2016;11(6):e0157130.
    Species: Human
    Sample Type: Whole Tissue
    Application: ELISA Capture
  2. A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases.
    Authors: Tabernero J, Elez M, Herranz M, Rico I, Prudkin L, Andreu J, Mateos J, Carreras M, Han M, Gifford J, Credi M, Yin W, Agarwal S, Komarnitsky P, Baselga J
    Clin Cancer Res, 2014;20(10):2793-804.
    Species: Human
    Sample Type: Serum
    Application: ELISA Capture
  3. The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling.
    Authors: Cen B, Xiong Y, Song J, Mahajan S, DuPont R, McEachern K, DeAngelo D, Cortes J, Minden M, Ebens A, Mims A, LaRue A, Kraft A
    Mol Cell Biol, 2014;34(13):2517-32.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Safety, pharmacokinetics, and pharmacodynamics of AMG 102, a fully human hepatocyte growth factor-neutralizing monoclonal antibody, in a first-in-human study of patients with advanced solid tumors.
    Authors: Gordon MS, Sweeney CS, Mendelson DS
    Clin. Cancer Res., 2010;16(2):699-710.
    Species: Human
    Sample Type: Plasma
    Application: Electrochemiluminescent Assay
  5. Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models.
    Authors: Coxon A, Rex K, Meyer S, Sun J, Sun J, Chen Q, Radinsky R, Kendall R, Burgess TL
    Mol. Cancer Ther., 2009;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: Electrochemiluminescence
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Staining Reagents
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Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
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Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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