Human HLA Class I PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7098P
Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (1)
FAQs
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Reviews (2)

Human HLA Class I PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
Source
Monoclonal Mouse IgG2A Clone # W6/32
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Membranes from human tonsillar lymphocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of HLA Class I antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I PE-conjugated Monoclonal Antibody (Catalog # FAB7098P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HLA Class I

HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.

References
  1. Barnstable, C.J. et al. (1978) Cell 14:9.
Long Name
Class I Human Leukocyte Antigen
Entrez Gene IDs
3105 (Human)
Alternate Names
A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLA Class I; HLAA; HLA-A; HLA-ABC; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80

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Citation for Human HLA Class I PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interference with pathways activated by topoisomerase inhibition alters the surface expression of PD-L1 and MHC I in colon cancer cells
    Authors: M Hassan, V Trung, D Bedi, S Shaddox, D Gunturu, C Yates, P Datta, T Samuel
    Oncology Letters, 2022-12-09;25(1):41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cyomtetry

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Reviews for Human HLA Class I PE-conjugated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human HLA Class I PE-conjugated Antibody
By Anonymous on 02/02/2023
Application: Flow Sample Tested: NK-92 human natural killer lymphoma cell line Species: Human

Human HLA Class I PE-conjugated Antibody
By Tahmineh Safaie on 11/08/2019
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse