Human HLA Class I PerCP-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7098C
Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry.
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Human HLA Class I PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
Source
Monoclonal Mouse IgG2A Clone # W6/32
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Membranes from human tonsillar lymphocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of HLA Class I antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I PerCP-conjugated Monoclonal Antibody (Catalog # FAB7098C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: HLA Class I

The MHC (Major Histocompatibility Complex) is a group of at least 200 genes located on chromosome 6 in human. It contains multiple groupings, one of which is called Class I that contains three distinct, but closely related molecules. These three molecules are known as MHC Class I-A, -B, and -C which, in the human, have been renamed HLA (Human Leukocyte Antigen)-A, -B, and -C. All are 44-46 kDa type I transmembrane glycoproteins that share approximately 85% amino acid sequence identity in their extracellular domains. And all represent the alpha - component of a alpha - beta heterodimer that utilizes the 11-12 kDa transmembrane beta 2-microglobulin protein as a beta -component. These HLA heterodimers appear on all nucleated cells, and serve as a platform for the presentation of cytoplasmic components (both self and foreign) to the alpha beta -TCR of cytotoxic CD8+ T cells. Unedited or mutated "self" components should be ignored, while tumor or viral components should elicit a cytotoxic immune response. This requires the continuous internal "processing" or degradation of large proteins into 8-10 amino acid peptides that are subsequently bound to a type A, B or C heterodimer and cycled to the plasma membrane. On the cell surface, the B chain is most common while the C chain is least common. And with advancing age, both the A and B chains decline in number. Not all A, B and C chains are "engaged"; while 90% of the HLA B chains are associated with antigen, only 30-70% of A and C chains are associated with processed antigens. And identical peptides can be perceived differently. For instance, a nine amino acid peptide with O-linked (but not N-linked) glycosylation will not be recognized by a CD8+ T cell that is specific for the naked nine amino acid peptide. Finally, the A, B and C chains, if not the entire heterodimeric complex, are now known to act in-cis with LILRB2, generating an activating complex on select cell types. The mouse MHC counterpart to the human HLA system is called H-2, and the two mouse genes that correspond to human HLA-A and -B show 68% amino acid sequence identity over their entire lengths.

Long Name
Class I Human Leukocyte Antigen
Entrez Gene IDs
3105 (Human)
Alternate Names
A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLA Class I; HLAA; HLA-A; HLA-ABC; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80

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