Detects the human major
histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A,
B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
Monoclonal Mouse IgG2A Clone # W6/32
Protein A or G purified from hybridoma culture supernatant
Membranes from human tonsillar lymphocytes
Supplied in a saline solution containing BSA and Sodium Azide.
PerCP (Peridinin-chlorophyll Protein Complex)
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I PerCP‑conjugated Monoclonal Antibody (Catalog # FAB7098C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: HLA Class I
The MHC (Major Histocompatibility Complex) is a group of at
least 200 genes located on chromosome 6 in human. It contains multiple groupings, one of which
is called Class I that contains three distinct, but closely related
molecules. These three molecules are
known as MHC Class I-A, -B, and -C which, in the human, have been renamed HLA
(Human Leukocyte Antigen)-A, -B, and -C.
All are 44-46 kDa type I transmembrane glycoproteins that share
approximately 85% amino acid sequence identity in their extracellular
domains. And all represent the alpha - component of a alpha - beta heterodimer that utilizes the 11-12 kDa transmembrane beta 2-microglobulin protein as a beta -component. These HLA heterodimers appear on
all nucleated cells, and serve as a platform for the presentation of
cytoplasmic components (both self and foreign) to the alpha beta -TCR of cytotoxic CD8+ T cells. Unedited or mutated "self" components should
be ignored, while tumor or viral components should elicit a cytotoxic immune
response. This requires the continuous
internal "processing" or degradation of large proteins into 8-10 amino acid
peptides that are subsequently bound to a type A, B or C heterodimer and cycled
to the plasma membrane. On the cell
surface, the B chain is most common while the C chain is least common. And with advancing age, both the A and B
chains decline in number. Not all A, B
and C chains are "engaged"; while 90% of the HLA B chains are associated with
antigen, only 30-70% of A and C chains are associated with processed
antigens. And identical peptides can be
perceived differently. For instance, a
nine amino acid peptide with O-linked (but not N-linked) glycosylation will not
be recognized by a CD8+ T cell that is specific for the naked nine amino acid
peptide. Finally, the A, B and C chains,
if not the entire heterodimeric complex, are now known to act in-cis with LILRB2, generating an
activating complex on select cell types. The mouse MHC counterpart to the human HLA
system is called H-2, and the two mouse genes that correspond to human HLA-A
and -B show 68% amino acid sequence identity over their entire lengths.
Class I Human Leukocyte Antigen
Entrez Gene IDs:
A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLAA; HLA-A; HLA-ABC; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.