Detects the human major
histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A,
B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
Monoclonal Mouse IgG2A Clone # W6/32
Protein A or G purified from hybridoma culture supernatant
Membranes from human tonsillar lymphocytes
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I APC‑conjugated Monoclonal Antibody (Catalog # FAB7098A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: HLA Class I
HLA-A, B, and C are approximately 45 kDa
transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They
contain three alpha domains in their extracellular regions. HLA molecules are expressed
on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This
complex binds peptides derived from pathogenic cytosolic or extracellular
proteins such as viral or microbial proteins. It presents these peptides on the
cell surface for recognition by the T cell receptor on CD8+ cytotoxic
T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched
MHC I alleles between a host and a donor lead to transplant rejection.
Barnstable, C.J. et al. (1978) Cell 14:9.
Class I Human Leukocyte Antigen
Entrez Gene IDs:
A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLAA; HLA-A; HLA-ABC; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.