Detects the human major
histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A,
B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
Monoclonal Mouse IgG2A Clone # W6/32
Protein A or G purified from hybridoma culture supernatant
Membranes from human tonsillar lymphocytes
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
0.25 µg/106 cells
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I Monoclonal Antibody (Catalog # MAB7098, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HLA Class I
HLA-A, B, and C are approximately 45 kDa
transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They
contain three alpha domains in their extracellular regions. HLA molecules are expressed
on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This
complex binds peptides derived from pathogenic cytosolic or extracellular
proteins such as viral or microbial proteins. It presents these peptides on the
cell surface for recognition by the T cell receptor on CD8+ cytotoxic
T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched
MHC I alleles between a host and a donor lead to transplant rejection.
Barnstable, C.J. et al. (1978) Cell 14:9.
Class I Human Leukocyte Antigen
Entrez Gene IDs:
A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLA Class I; HLAA; HLA-A; HLA-ABC; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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