Detects the human major
histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A,
B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
Monoclonal Mouse IgG2A Clone # W6/32
Protein A or G purified from hybridoma culture supernatant
Membranes from human tonsillar lymphocytes
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
0.25 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I Monoclonal Antibody (Catalog # MAB7098, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HLA Class I
HLA-A, B, and C are approximately 45 kDa
transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They
contain three alpha domains in their extracellular regions. HLA molecules are expressed
on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This
complex binds peptides derived from pathogenic cytosolic or extracellular
proteins such as viral or microbial proteins. It presents these peptides on the
cell surface for recognition by the T cell receptor on CD8+ cytotoxic
T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched
MHC I alleles between a host and a donor lead to transplant rejection.
Barnstable, C.J. et al. (1978) Cell 14:9.
Class I Human Leukocyte Antigen
Entrez Gene IDs:
A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLAA; HLA-A; HLA-ABC; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.