Human HLA Class I Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
  • Source
    Monoclonal Mouse IgG2A Clone # W6/32
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Membranes from human tonsillar lymphocytes
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-reported
    This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I Monoclonal Antibody (Catalog # MAB7098, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HLA Class I
HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.
  • References:
    1. Barnstable, C.J. et al. (1978) Cell 14:9.
  • Long Name:
    Class I Human Leukocyte Antigen
  • Entrez Gene IDs:
    3105 (Human)
  • Alternate Names:
    A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLA Class I; HLAA; HLA-A; HLA-ABC; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
    Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
    Sci Rep, 2017;7(1):8661.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Involvement of the TNF-alpha/TGF-beta/IDO axis in IVIg-induced immune tolerance.
    Authors: Loubaki L, Chabot D, Bazin R
    Cytokine, 2015;71(2):181-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012;8(1):e1002486.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Mouse IgG2A Antibody

Flow MAB0032  
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