Human HNF-3 alpha /FoxA1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human HNF‑3 alpha /FoxA1 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 654126
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human HNF‑3 alpha /FoxA1
    Met253-Ser472
    Accession # P55317
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    1-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human HNF‑3 alpha /FoxA1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 30 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human HNF‑3 alpha /FoxA1 Monoclonal Antibody (Catalog # MAB6778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HNF‑3 alpha /FoxA1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
HNF‑3 alpha /FoxA1 in MCF‑7 Human Cell Line. HNF‑3 alpha /FoxA1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human HNF‑3 alpha /FoxA1 Monoclonal Antibody (Catalog # MAB6778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
HNF‑3 alpha /FoxA1 in Human Breast Cancer Tissue. HNF‑3 alpha /FoxA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human HNF‑3 alpha /FoxA1 Monoclonal Antibody (Catalog # MAB6778) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HNF-3 alpha/FoxA1
FoxA1 (forkhead box A1), also called HNF-3 alpha (hepatocyte nuclear factor 3 alpha) or TCF3A (transcription factor 3A) is a 473 amino acid (aa), 49 kDa (predicted) protein of the forkhead nuclear transcription factor family. It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors. FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines. Within the sequence used as an immunogen, human FoxA1 shares 93% and 86% aa identity with mouse and rat FoxA1, respectively.
  • Long Name:
    Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1
  • Entrez Gene IDs:
    3169 (Human); 15375 (Mouse); 25098 (Rat)
  • Alternate Names:
    forkhead box A1; Forkhead box protein A1; FoxA1; hepatocyte nuclear factor 3, alpha; hepatocyte nuclear factor 3-alpha; HNF3 alpha; HNF-3 alpha; HNF-3A; HNF-3-alpha; HNF3ATCF-3A; MGC33105; TCF3A; Transcription factor 3A
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