Human ICAM-1/CD54 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF720
Product Details
Citations (5)
FAQs
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Human ICAM-1/CD54 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with rhICAM-2 and rhICAM-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ICAM‑1/CD54 (Catalog # ADP4)

Human ICAM-1/CD54 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human ICAM‑1/CD54 Antibody (Catalog # MAB720)

Standard: Recombinant Human ICAM-1/CD54 Protein, CF (Catalog # ADP4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Product Datasheets

Citations for Human ICAM-1/CD54 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. High plasma levels of soluble intercellular adhesion molecule (ICAM)-1 are associated with cerebral malaria.
    Authors: Adukpo, Selorme, Kusi, Kwadwo A, Ofori, Michael, Tetteh, John K A, Amoako-Sakyi, Daniel, Goka, Bamenla, Adjei, George O, Edoh, Dominic, Akanmori, Bartholo, Gyan, Ben A, Dodoo, Daniel
    PLoS ONE, 2013;8(12):e84181.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  2. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
    Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
    Thromb. Haemost., 2011;107(1):140-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  3. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  4. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  5. Opposing roles of blood myeloid and plasmacytoid dendritic cells in HIV-1 infection of T cells: transmission facilitation versus replication inhibition.
    Authors: Groot F, van Capel TM, Kapsenberg ML, Berkhout B, de Jong EC
    Blood, 2006;108(6):1957-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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