Human ICAM-1/CD54 Antibody

Catalog # Availability Size / Price Qty
MAB720-SP
MAB720-100
MAB720-500
Human ICAM-1/CD54 Antibody in Immunocytochemistry
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)

Human ICAM-1/CD54 Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human (rh)ICAM-1 in direct ELISAs and Western blots. In Western blots, shows no cross-reactivity with rrICAM-1, rhICAM-2, rhICAM-3, rhVCAM-1, and rmDCC.
Source
Monoclonal Mouse IgG1 Clone # 14C11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ICAM‑1/CD54 (Catalog # ADP4)
under non-reducing conditions only
Immunocytochemistry
5-25 µg/mL
See below

Human ICAM-1/CD54 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human ICAM‑1/CD54 Biotinylated Antibody (Catalog # BAF720)

Standard: Recombinant Human ICAM-1/CD54 Protein, CF (Catalog # ADP4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry ICAM‑1/CD54  in A431 Human Cell Line. View Larger

ICAM‑1/CD54 in A431 Human Cell Line. ICAM‑1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human ICAM‑1/CD54 Monoclonal Antibody (Catalog # MAB720) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be upregulated upon cell activation.

Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Product Datasheets

Citations for Human ICAM-1/CD54 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. High plasma levels of soluble intercellular adhesion molecule (ICAM)-1 are associated with cerebral malaria.
    Authors: Adukpo, Selorme, Kusi, Kwadwo A, Ofori, Michael, Tetteh, John K A, Amoako-Sakyi, Daniel, Goka, Bamenla, Adjei, George O, Edoh, Dominic, Akanmori, Bartholo, Gyan, Ben A, Dodoo, Daniel
    PLoS ONE, 2013;8(12):e84181.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  2. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  3. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human ICAM-1/CD54 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human ICAM-1/CD54 Antibody
By Anonymous on 07/23/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was successfully employed in a competitive immunoassay to measure serum ICAM-1 levels.


Human ICAM-1/CD54 Antibody
By Anonymous on 09/05/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human