Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Immunocytochemistry

Cited:

ELISA Development, ELISA Microarray Development, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 14C11
View all ICAM-1/CD54 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
Extracellular domain

Specificity

Detects recombinant human (rh)ICAM-1 in direct ELISAs and Western blots. In Western blots, shows no cross-reactivity with rrICAM-1, rhICAM-2, rhICAM-3, rhVCAM-1, and rmDCC.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ICAM‑1/CD54 Antibody

ICAM-1/CD54 antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

ICAM‑1/CD54 in A431 Human Cell Line.

ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB720) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human ICAM‑1/CD54 Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human ICAM‑1/CD54 (Catalog # ADP4)
under non-reducing conditions only

Human ICAM-1/CD54 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human ICAM‑1/CD54 Biotinylated Antibody (Catalog # BAF720)
  • Standard: Recombinant Human ICAM-1/CD54 Protein, CF (Catalog # ADP4)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB720 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be upregulated upon cell activation.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

3383 (Human); 15894 (Mouse); 25464 (Rat)

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for Human ICAM‑1/CD54 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ICAM‑1/CD54 Antibody

For research use only

Citations for Human ICAM‑1/CD54 Antibody

Customer Reviews for Human ICAM‑1/CD54 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human ICAM-1/CD54 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: endothelial cells
    Species: Human
    Verified Customer | Posted 11/05/2021
    Human ICAM‑1/CD54 Antibody MAB720
  • Human ICAM-1/CD54 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/23/2019
    This antibody was successfully employed in a competitive immunoassay to measure serum ICAM-1 levels.
    Human ICAM‑1/CD54 Antibody MAB720
  • Human ICAM-1/CD54 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 09/05/2018
    Human ICAM‑1/CD54 Antibody MAB720

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Protocols

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