Human IFN-alpha Antibody

Catalog # Availability Size / Price Qty
31101-1

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Product Details
Citations (2)
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Human IFN-alpha Antibody Summary

Species Reactivity
Human
Specificity
Human IFN-alpha
Source
Polyclonal Rabbit Serum
Purification
N/A
Immunogen
E. coli-derived recombinant human IFN-alpha
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using A549 cells and EMCV was used as the challenge virus [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [Gxa01-901-535]. This material is prepared specifically for effective neutralization of Hu-IFN-alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For
long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the
contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers
containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-alpha

The most common use of this product has been in a pool with Rabbit Anti-Human Interferon Beta (PBL # 31410) and Mouse Anti-IFNAR2 (PBL# 21385) to block the activity of all Type I IFNs. Krug et al [Eur. J. Immunol. (2001) 31:2154] used a pool of PBL #31101, #31410 and #21385 to demonstrate PBMC-derived Type I IFN is responsible for increased IFN-gamma secretion after stimulation with CpG ODN. Ito et al. [J. Exp. Med. (2002) 195:1507] used a similar pool to determine that TLR7 stimulated pDC but not mDC induce Th1 development through Type I IFN. Lande et al. [J. Immunol. (2003) 170:1174] used a pool of PBL#31410 and #31101 to show a blockade of autocrine Alpha/Beta action leads to decreased CXCL-10 secretion from Mycobacterium-infected dendritic cells. Jego et al. [Immunity (2003) 19:225] demonstrated the inhibition of IFN-alpha signaling in a co-culture of pDCs with CD40 stimulated B-cells reduces IgG secretion, while inhibition of IFN-beta signaling does not occur. Ku et al. [J. Exp. Med. (2004) 200:917] showed epithelial cells surrounding Varicella Zoster lesions express IFN-alpha by immunohistochemistry (IHC) in a SCID-human skin zenograft model.

Long Name
Interferon alpha
Entrez Gene IDs
3440 (Human)
Alternate Names
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

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Citations for Human IFN-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Targeting transgenic proteins to alpha granules for platelet-directed gene therapy
    Authors: Vanessa M.A. Woods, Lisette J. Latorre-Rey, Franziska Schenk, Marcel G.E. Rommel, Thomas Moritz, Ute Modlich
    Molecular Therapy - Nucleic Acids
  2. TLR4 ligands induce IFN-alpha production by mouse conventional dendritic cells and human monocytes after IFN-beta priming.
    Authors: Richez C, Yasuda K, Watkins AA
    J. Immunol., 2009-01-15;182(2):820-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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