IFN-beta (Interferon-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
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Human IFN-beta Quantikine ELISA Kit
R&D Systems | Catalog # DIFNB0
Quantikine Quickit version available - results in 90 minutes (QK410).
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL)
Sensitivity
0.781 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates)
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Product Summary for Human IFN-beta Quantikine ELISA Kit
The Quantikine® Human IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IFN-beta in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human IFN-beta.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Cell Culture Supernates:
A549 human lung carcinoma cells were cultured in Kaighn's Nutrient Mixture F-12
supplemented with 10% fetal bovine serum until nearly confluent. The cells were cultured
unstimulated or stimulated with 10 μg/mL of poly I:C in the presence of Lipofectamine 2000
(LF2K) for 24 hours. Aliquots of the cell culture supernates were removed and assayed for levels
of human IFN-beta.
ND=Non-detectable
ND=Non-detectable
| Condition | pg/mL |
| Unstimulated | ND |
| Stimulated | 848 |
CCD-1070Sk human foreskin fibroblast cells were cultured in MEM NEAA Earle's Salts
supplemented with 10% fetal bovine serum until nearly confluent. The cells were cultured
unstimulated or stimulated with 10 μg/mL of poly I:C in the presence of Lipofectamine 2000
(LF2K) for 24 hours. Aliquots of the cell culture supernates were removed and assayed for levels
of human IFN-beta.
| Condition | pg/mL |
| Unstimulated | ND |
| Stimulated | 2323 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 58.3 | 159 | 295 | 56.4 | 160 | 275 |
| Standard Deviation | 1.71 | 6.01 | 13.8 | 5.26 | 13.0 | 21.9 |
| CV% | 2.9 | 3.8 | 4.7 | 9.3 | 8.1 | 8.0 |
Recovery for Human IFN-beta Quantikine ELISA Kit
The recovery of human IFN-beta spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 107 | 95-111 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IFN-beta Quantikine ELISA Kit
Human IFN-beta Quantikine ELISA Spiked Recovery Competitor Comparision
IFN-beta is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. R&D Systems recovery is 98% compared to 55% for the top competitor.
Human IFN-beta Quantikine ELISA Natural Linearity Competitor Comparison
Samples containing high concentrations of IFN-beta in various matrices are diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. R&D Systems linearity is between 103%-112% compared to 119%-170% for the top competitor.
Human IFN- beta ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-beta
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
Additional IFN-beta Products
Product Documents for Human IFN-beta Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IFN-beta Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human IFN-beta Quantikine ELISA Kit
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Customer Reviews for Human IFN-beta Quantikine ELISA Kit (6)
4.8 out of 5
6 Customer Ratings
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Sample Tested: Cell Culture SupernatesVerified Customer | Posted 01/02/2025
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Sample Tested: Cell culture supernatantVerified Customer | Posted 01/02/2025
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Sample Tested: Lung cancer tissue, HCC827 human non-small cell lung cancer cell line and A549 human lung carcinoma cell lineVerified Customer | Posted 10/14/2023The test kit's results are as expected! We are very excited. We examined various lung cancer cell lines for immune cell interventions and the TME components of primary cultured tissues.
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Sample Tested: Blood B cellsVerified Customer | Posted 10/18/2020
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Sample Tested: Monocyte-derived dendritic cellsVerified Customer | Posted 12/18/2019Human Monocyte-Derived Dendritic cells infected with a lentivirus. 2x diluted cell culture supernatants.
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Sample Tested: Jurkat human acute T cell leukemia cell lineVerified Customer | Posted 08/06/2019
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