Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Flow Cytometry Control

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 33255
View all IGF-I R/IGF1R Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R
Glu31-Asn932
Accession # P08069

Specificity

Detects human IGF-I R in sandwich immunoassays and Western blots. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL-9 R, and rhTGF-beta  RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IGF-I R/IGF1R Fluorescein‑conjugated Antibody

Detection of IGF‑I R antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry.

Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB391F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IGF-I R/IGF1R Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line

Reviewed Applications

Read 1 review rated 5 using FAB391F in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IGF-I R/IGF1R

Insulin-like Growth Factor I Receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

UniProt

P08069

Additional IGF-I R/IGF1R Products

Product Documents for Human IGF-I R/IGF1R Fluorescein‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IGF-I R/IGF1R Fluorescein‑conjugated Antibody

For research use only

Citations for Human IGF-I R/IGF1R Fluorescein‑conjugated Antibody

Customer Reviews for Human IGF-I R/IGF1R Fluorescein‑conjugated Antibody (1)

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  • Human IGF-I R/IGF1R Fluorescein-conjugated Antibody
    Name: Vincent Giacalone
    Application: Flow Cytometry
    Sample Tested: Peripheral blood monocytes
    Species: Human
    Verified Customer | Posted 07/21/2020
    Human peripheral blood was stained and analyzed by flow cytometry. Monocytes were identified among CD45+ leukocytes and analyzed for IGF-IR expression. This antibody achieves reliable fluorescence in the FITC channel
    Human IGF-I R/IGF1R Fluorescein‑conjugated Antibody FAB391F

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