Human IGF-I R/IGF1R Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB391F-100
FAB391F-025
Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human IGF-I R/IGF1R Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGF-I R in sandwich immunoassays and Western blots. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL-9 R, and rhTGF-beta  RII.
Source
Monoclonal Mouse IgG1 Clone # 33255
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R
Glu31-Asn932
Accession # P08069
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry. View Larger

Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB391F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IGF-I R/IGF1R

Insulin-like Growth Factor I Receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

Long Name
Insulin-like Growth Factor I Receptor
Entrez Gene IDs
3480 (Human)
Alternate Names
CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

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Citations for Human IGF-I R/IGF1R Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Insulin-like growth factor 1 receptor and response to anti-IGF1R antibody therapy in osteosarcoma.
    Authors: Cao, Yu, Roth, Michael, Piperdi, Sajida, Montoya, Kristofe, Sowers, Rebecca, Rao, Pulivart, Geller, David, Houghton, Peter, Kolb, E Anders, Gill, Jonathan, Gorlick, Richard
    PLoS ONE, 2014;9(8):e106249.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  2. Growth hormone-induced stimulation of multilineage human hematopoiesis.
    Authors: Hanley MB, Napolitano LA, McCune JM
    Stem Cells, 2005;23(8):1170-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human IGF-I R/IGF1R Fluorescein-conjugated Antibody

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Human IGF-I R/IGF1R Fluorescein-conjugated Antibody
By Vincent Giacalone on 07/21/2020
Application: Flow Sample Tested: Peripheral blood monocytes Species: Human

Human peripheral blood was stained and analyzed by flow cytometry. Monocytes were identified among CD45+ leukocytes and analyzed for IGF-IR expression. This antibody achieves reliable fluorescence in the FITC channel