Human IGF-I R/IGF1R PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB391P-100
FAB391P-025
Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry.
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Product Details
Citations (5)
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Human IGF-I R/IGF1R PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGF-I R in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL-9 R, and rhTGF-beta  RII.
Source
Monoclonal Mouse IgG1 Clone # 33255
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R
Glu31-Asn932
Accession # P08069
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IGF-I R antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R PE-conjugated Monoclonal Antibody (Catalog # FAB391P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IGF-I R/IGF1R

Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

Long Name
Insulin-like Growth Factor I Receptor
Entrez Gene IDs
3480 (Human)
Alternate Names
CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

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Citations for Human IGF-I R/IGF1R PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Quantification of IGF-1 Receptor May Be Useful in Diagnosing Polycythemia Vera-Suggestion to Be Added to Be One of the Minor Criterion
    PLoS ONE, 2016-11-03;11(11):e0165299.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
    Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
    Br J Cancer, 2014-05-22;110(12):2865-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
    Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
    PLoS ONE, 2014-01-29;9(1):e84417.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Clinical and functional characterization of a patient carrying a compound heterozygous pericentrin mutation and a heterozygous IGF1 receptor mutation.
    Authors: Muller E, Dunstheimer D, Klammt J, Friebe D, Kiess W, Kratzsch J, Kruis T, Laue S, Pfaffle R, Wallborn T, Heidemann PH
    PLoS ONE, 2012-05-31;7(5):e38220.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Insulin-like growth factor I (IGF-I) stimulates proliferation but also increases caspase-3 activity, Annexin-V binding, and DNA-fragmentation in human MG63 osteosarcoma cells: co-activation of pro- and anti-apoptotic pathways by IGF-I.
    Authors: Raile K, Hille R, Laue S, Schulz A, Pfeifer G, Horn F, Kiess W
    Horm. Metab. Res., 2003-11-01;35(11):786-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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