Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Bovine

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Flow Cytometry, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Capture, ELISA Development, In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 33255
View all IGF-I R/IGF1R Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R/IGF1R
Glu31-Asn932
Accession # P08069

Specificity

Detects human IGF-I R/IGF1R in sandwich ELISAs and Western blots. Detects mouse IGF-I R/IGF1R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF‑II, rhIL‑3 R alpha, rhIL‑9 R, and rhTGF‑ beta  RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human/Mouse IGF-I R/IGF1R Antibody

Detection of Human IGF-I R/IGF1R antibody by Western Blot.

Detection of Human IGF-I R/IGF1R by Western Blot.

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, SK-Mel-28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGF-I R/IGF1R at approximately 275 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.
Detection of IGF-I R/IGF1R antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of IGF-I R/IGF1R in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
IGF-I R/IGF1R antibody in MCF-7 and HDLM Human Cell Lines by Immunocytochemistry (ICC).

IGF-I R/IGF1R in MCF‑7 and HDLM Human Cell Lines.

IGF-I R/IGF1R was detected in immersion fixed MCF-7 human breast cancer cell line (positive staining; left panel) and HDLM human Hodgkin's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane in MCF-7 cell line. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IGF-I R/IGF1R antibody in Mouse Heart by Immunohistochemistry (IHC-P).

IGF-I R/IGF1R in Mouse Heart.

IGF-I R/IGF1R was detected in perfusion fixed paraffin-embedded sections of mouse heart using Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF-I R/IGF1R Antibody.

Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF-I R/IGF1R Antibody.

Recombinant Human (rh) IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by rhIGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391). At 11 µg/mL, this antibody will neutralize 50-75% rhIGF-1 induced activity.
Detection of IGF-I R/IGF1R by Immunocytochemistry/ Immunofluorescence

Detection of IGF-I R/IGF1R by Immunocytochemistry/ Immunofluorescence

IGF1-R inhibition decreases autophagosome precursor formation by reducing clathrin-dependent endocytosis. (D–F) HeLa cells were transfected with 0.5 μg of the GFP-Atg16L1 construct. After 24 h, the cells were treated for 24 h with MAB391, fixed&analysed under fluorescence microscope. The P-values for assessing the %age of cells showing ≥20 GFP-Atg16-positive vesicles as well as the number of GFP-Atg16 vesicles/cell determined by Student's t-test (E: n = 3; CTRL versus 24 h MAB391, P = 0.00272; F: n = 3; CTRL versus 24 h MAB391, P = 0.00496). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23804751), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse IGF-I R/IGF1R Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: MCF-7 human breast cancer cell line

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Immunohistochemistry

5-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse heart

Western Blot

1 µg/mL
Sample: NTera-2 human testicular embryonic carcinoma cell line, SK-Mel-28 human malignant melanoma cell line, and G361 human melanoma cell line under non-reducing conditions only

Neutralization

Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. At 11 µg/mL, this antibody will neutralize approximately 50-75% of the bioactivity due to 6 ng/mL Recombinant Human IGF‑I.

Human IGF-I R/IGF1R Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IGF-I R/IGF1R Biotinylated Antibody (Catalog # BAF391)
  • Standard: Recombinant Human IGF-I R/IGF1R Protein, CF (Catalog # 391-GR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB391 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I R/IGF1R

Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

UniProt

P08069

Additional IGF-I R/IGF1R Products

Product Documents for Human/Mouse IGF-I R/IGF1R Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse IGF-I R/IGF1R Antibody

For research use only

Citations for Human/Mouse IGF-I R/IGF1R Antibody

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  • Human/Mouse IGF-I R/IGF1R Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Heart tissue
    Species: Mouse
    Verified Customer | Posted 05/20/2022
    Human/Mouse IGF-I R/IGF1R Antibody MAB391

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