Human IGFBP-rp1/IGFBP-7 Antibody

Catalog # Availability Size / Price Qty
AF1334
AF1334-SP
Human IGFBP-rp1/IGFBP-7 Antibody in Western Blot
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Product Details
Citations (5)
FAQs
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Human IGFBP-rp1/IGFBP-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP-rp1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) IGFBP‑L1, rhIGFBP-rp10 and recombinant mouse IGFBP-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑rp1
Arg98-Arg277
Accession # AAA16187
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human IGFBP‑rp1/IGFBP‑7 by Western Blot. View Larger

Detection of Human IGFBP‑rp1/IGFBP‑7 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IGFBP‑rp1/IGFBP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFBP‑rp1/IGFBP‑7 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IGFBP‑rp1/IGFBP‑7 in Human Pancreas. View Larger

IGFBP‑rp1/IGFBP‑7 in Human Pancreas. IGFBP‑rp1/IGFBP‑7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human IGFBP‑rp1/IGFBP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-rp1/IGFBP-7

IGFBP-rp1, also known as Mac25/Angiomodulin (AGM), tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. Mature IGFBP-rp1 is a glycosylated protein with an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal immunoglobulin-like C2-type domain. The similarity of IGFBP-rp1 with the IGFBPs is confined to the N-terminal IGFBP domain, which contains all 12 of the conserved cysteine residues found in IGFBP-1 through 5. Human and mouse IGFBP-rp1 are highly homologous. Discounting a segment of 43 aa near the N-terminus that is missing in the mouse homologue, human and mouse IGFBP-rp1 share 94% aa sequence identity. IGFBP-rp1 is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. The expression of IGFBP-rp1 is upregulated in senescing epithelial cells and by retinoic acid. IGFBP-rp1 binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP-rp1 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. IGFBP‑rp1 supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI2. It modulates tumor cell growth and has also been implicated in angiogenesis. IGFBP-rp1 is proteolytically cleaved between lysine 97 and alanine 98. Cleaved IGFBP-rp1 has enhanced cell attachment activity but can no longer bind IGF/insulin (1-3).

References
  1. Hwa, V. et al. (1999) Endocrinology Rev. 20:761.
  2. Nagakubo, D. et al. (2003) J. Immunol. 171:553.
  3. Ahmed, S. et al. (2003) Biochem. Biophys. Res. Commun. 310:612.
Long Name
Insulin-like Growth Factor Binding Protein Related Protein 1
Entrez Gene IDs
3490 (Human); 29817 (Mouse)
Alternate Names
angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7; IGFBP-7PGI2-stimulating factor; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; insulin-like growth factor-binding protein 7; MAC25 protein; Mac25; MAC25IGF-binding protein 7; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor

Product Datasheets

Citations for Human IGFBP-rp1/IGFBP-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. IGFBP7, a novel tumor stroma marker, with growth-promoting effects in colon cancer through a paracrine tumor-stroma interaction.
    Authors: Rupp C, Scherzer M, Rudisch A, Unger C, Haslinger C, Schweifer N, Artaker M, Nivarthi H, Moriggl R, Hengstschlager M, Kerjaschki D, Sommergruber W, Dolznig H, Garin-Chesa P
    Oncogene, 2015;34(7):815-25.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. Disulfiram suppresses growth of the malignant pleural mesothelioma cells in part by inducing apoptosis.
    Authors: Cheriyan V, Wang Y, Muthu M, Jamal S, Chen D, Yang H, Polin L, Tarca A, Pass H, Dou Q, Sharma S, Wali A, Rishi A
    PLoS ONE, 2014;9(4):e93711.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
    Authors: Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ
    J. Invest. Dermatol., 2010;130(6):1685-96.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Insulin-like growth factor binding protein 7 mediates glioma cell growth and migration.
    Authors: Jiang W, Xiang C, Cazacu S, Brodie C, Mikkelsen T
    Neoplasia, 2008;10(12):1335-42.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Glioblastoma-secreted factors induce IGFBP7 and angiogenesis by modulating Smad-2-dependent TGF-beta signaling.
    Authors: Pen A, Moreno MJ, Durocher Y, Deb-Rinker P, Stanimirovic DB
    Oncogene, 2008;27(54):6834-44.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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