Human IGFBP-rp1/IGFBP-7 Antibody

Detection of Human IGFBP‑rp1/IGFBP‑7 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IGFBP-rp1/IGFBP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFBP-rp1/IGFBP-7 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IGFBP‑rp1/IGFBP‑7 in Human Pancreas. IGFBP-rp1/IGFBP-7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human IGFBP-rp1/IGFBP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of IGFBP-rp1/IGFBP-7 by Western Blot IGFBP7 expression is increased in human ASCs going through differentiation with TGF-beta and BMP-4. (A) Top is representative immunoblot for IGFBP7 expression. Corresponding line graph demonstrating the time-course (0 to 7 days) effect of TGF-beta and BMP-4 on IGFBP7 expression. TGF-beta and BMP-4 synergistically induce the expression of IGFBP7 on hASC. hASCs were stimulated with TGF-beta, BMP-4 or combination of TGF-beta and BMP-4 (DM) for up to 7 days and IGFBP7 expression was evaluated by Western blot. Top are representative immunoblots for IGFBP7 expression. Corresponding bar graph demonstrating the time-course (0 to 7 days) effect of TGF-beta, BMP-4 or DM on IGFBP7 expression. (B) Fluorescence microscopy was also used to detect TGF-beta, BMP-4 or DM-induced IGFBP7 expression on day 0, 4 and 7. Scale bar 100µm. Results are mean ± SEM of 5 experiments. * p < 0.05 vs. day 0 of stimulation with DM; ** p < 0.05 vs. day 0 of stimulation with TGF-beta ; # p < 0.05 vs. day 0 of stimulation with BMP-4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34356861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IGFBP-rp1/IGFBP-7 by Immunocytochemistry/ Immunofluorescence IGFBP7 expression is increased in human ASCs going through differentiation with TGF-beta and BMP-4. (A) Top is representative immunoblot for IGFBP7 expression. Corresponding line graph demonstrating the time-course (0 to 7 days) effect of TGF-beta and BMP-4 on IGFBP7 expression. TGF-beta and BMP-4 synergistically induce the expression of IGFBP7 on hASC. hASCs were stimulated with TGF-beta, BMP-4 or combination of TGF-beta and BMP-4 (DM) for up to 7 days and IGFBP7 expression was evaluated by Western blot. Top are representative immunoblots for IGFBP7 expression. Corresponding bar graph demonstrating the time-course (0 to 7 days) effect of TGF-beta, BMP-4 or DM on IGFBP7 expression. (B) Fluorescence microscopy was also used to detect TGF-beta, BMP-4 or DM-induced IGFBP7 expression on day 0, 4 and 7. Scale bar 100µm. Results are mean ± SEM of 5 experiments. * p < 0.05 vs. day 0 of stimulation with DM; ** p < 0.05 vs. day 0 of stimulation with TGF-beta ; # p < 0.05 vs. day 0 of stimulation with BMP-4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34356861), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IGFBP-rp1/IGFBP-7 by Western Blot Committed Cardiac Progenitors and their Signature Genes.a Schematic showing the laminin (LN) protocol that uses LN-521 + 221 as a defined extracellular matrix together with small molecule inhibitors for the differentiation of pluripotent stem cells to day 11 committed cardiac progenitors (CCPs). b Heat map of three biological replicates from bulk RNA-seq data demonstrating the canonical progenitor gene expression on days 9 and 11. Supplementary Table 1 shows the actual transcripts per million (TPM) for each gene. c Time course analysis of scRNAseq data from days 0 to 11 for known canonical gene expression: PDGFRA, GATA4, KDR, NKX2–5, ISL1, and MESP1. d Immunoblotting of canonical progenitor genes from differentiation days 7, 9, and 11. Supplementary Fig. 1 shows the quantification of each blot from five biological replicates. e Heat map of the bulk RNA-seq data using CCP signature genes at differentiation days 9 and 11. Supplementary Table 1 shows the actual TPM for each gene. f Single-cell RNA-seq data (two biological replicates) of the CCP signature gene expression in tSNE plots from days 0, 4, 7, 9, and 11. The tSNE plot at the top left shows the overall distribution of each day using different colors. g Time course analysis of scRNAseq data from days 0 to 11 for day 11 CCP gene signature markers. h Immunoblotting of CCP signature genes from differentiation days 7, 9, and 11 (5 biological replicates). Supplementary Fig. 1 shows the quantification of each blot. Comparisons between groups were performed using two-way ANOVA with Tukey post hoc analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37236990), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IGFBP-rp1/IGFBP-7 by Western Blot Committed Cardiac Progenitors and their Signature Genes.a Schematic showing the laminin (LN) protocol that uses LN-521 + 221 as a defined extracellular matrix together with small molecule inhibitors for the differentiation of pluripotent stem cells to day 11 committed cardiac progenitors (CCPs). b Heat map of three biological replicates from bulk RNA-seq data demonstrating the canonical progenitor gene expression on days 9 and 11. Supplementary Table 1 shows the actual transcripts per million (TPM) for each gene. c Time course analysis of scRNAseq data from days 0 to 11 for known canonical gene expression: PDGFRA, GATA4, KDR, NKX2–5, ISL1, and MESP1. d Immunoblotting of canonical progenitor genes from differentiation days 7, 9, and 11. Supplementary Fig. 1 shows the quantification of each blot from five biological replicates. e Heat map of the bulk RNA-seq data using CCP signature genes at differentiation days 9 and 11. Supplementary Table 1 shows the actual TPM for each gene. f Single-cell RNA-seq data (two biological replicates) of the CCP signature gene expression in tSNE plots from days 0, 4, 7, 9, and 11. The tSNE plot at the top left shows the overall distribution of each day using different colors. g Time course analysis of scRNAseq data from days 0 to 11 for day 11 CCP gene signature markers. h Immunoblotting of CCP signature genes from differentiation days 7, 9, and 11 (5 biological replicates). Supplementary Fig. 1 shows the quantification of each blot. Comparisons between groups were performed using two-way ANOVA with Tukey post hoc analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37236990), licensed under a CC-BY license. Not internally tested by R&D Systems.