Human IGFBP-rP10 Antibody

Catalog # Availability Size / Price Qty
AF5137
AF5137-SP
Detection of Human IGFBP-rP10 by Western Blot.
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Human IGFBP-rP10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP-rP10 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse IGFBP-rP10 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑rP10 isoform 1
Arg31-Tyr304
Accession # Q96I82
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human IGFBP-rP10 by Western Blot. View Larger

Detection of Human IGFBP-rP10 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti‑Human IGFBP-rP10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5137) followed by HRP-conjugated Anti‑Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IGFBP-rP10 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-rP10

IGFBP-rP10 (IGF binding protein-related protein 10; also Kazal-type serine protease inhibitor domain-contain protein 1 and BONO1) is a 37 kDa (predicted), secreted member of the IGFBP superfamily. It is expressed by osteoblasts and odontoblasts, is associated with regions of mineralization, and stimulates the proliferation of osteoblasts in vitro. Mature human IGFBP-rP10 is 274 amino acids (aa) in length. It contains an IGF binding domain (aa 49‑124), a follistatin-like serine protease inhibitor region (aa 125‑168) and a C2-type Ig‑like domain (aa 172‑269). There is one splice variant that shows a 70 aa substitution for aa 184‑304. Mature human IGFBP‑rP10 is 87% aa identical to mouse IGFBP-rP10.

Long Name
Insulin-like Growth Factor Binding Protein Related Protein-10
Entrez Gene IDs
81621 (Human); 107250 (Mouse); 293997 (Rat)
Alternate Names
bone- and odontoblast-expressed gene 1; Bono1; FKSG28; FKSG40; FLJ24094; IGFBP-related protein 10; IGFBPrP10; IGFBP-rP10; KAZALD1; Kazal-type serine peptidase inhibitor domain 1; Kazal-type serine protease inhibitor domain 1; kazal-type serine protease inhibitor domain-containing protein 1; novel kazal-type serine protease inhibitor domain and immunoglobulin domain containing protein; UNQ2945/PRO21184

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