Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti‑Human IGFBP-rP10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5137) followed by HRP-conjugated Anti‑Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IGFBP-rP10 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IGFBP-rP10 (IGF binding protein-related protein 10; also Kazal-type serine protease inhibitor domain-contain protein 1 and BONO1) is a 37 kDa (predicted), secreted member of the IGFBP superfamily. It is expressed by osteoblasts and odontoblasts, is associated with regions of mineralization, and stimulates the proliferation of osteoblasts in vitro. Mature human IGFBP-rP10 is 274 amino acids (aa) in length. It contains an IGF binding domain (aa 49‑124), a follistatin-like serine protease inhibitor region (aa 125‑168) and a C2-type Ig‑like domain (aa 172‑269). There is one splice variant that shows a 70 aa substitution for aa 184‑304. Mature human IGFBP‑rP10 is 87% aa identical to mouse IGFBP-rP10.
Insulin-like Growth Factor Binding Protein Related Protein-10
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