Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all IGFBP-rP10 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IGFBP‑rP10 isoform 1
Arg31-Tyr304
Accession # Q96I82

Specificity

Detects human IGFBP-rP10 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse IGFBP-rP10 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human IGFBP‑rP10 Antibody

Detection of Human IGFBP-rP10 antibody by Western Blot.

Detection of Human IGFBP-rP10 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IGFBP-rP10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5137) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IGFBP-rP10 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human IGFBP‑rP10 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGFBP-rP10

IGFBP-rP10 (IGF binding protein-related protein 10; also Kazal-type serine protease inhibitor domain-contain protein 1 and BONO1) is a 37 kDa (predicted), secreted member of the IGFBP superfamily. It is expressed by osteoblasts and odontoblasts, is associated with regions of mineralization, and stimulates the proliferation of osteoblasts in vitro. Mature human IGFBP-rP10 is 274 amino acids (aa) in length. It contains an IGF binding domain (aa 49‑124), a follistatin-like serine protease inhibitor region (aa 125‑168) and a C2-type Ig‑like domain (aa 172‑269). There is one splice variant that shows a 70 aa substitution for aa 184‑304. Mature human IGFBP‑rP10 is 87% aa identical to mouse IGFBP-rP10.

Long Name

Insulin-like Growth Factor Binding Protein Related Protein-10

Alternate Names

Bono1, FKSG28, FKSG40, IGFBPrP10, KAZALD1

Entrez Gene IDs

81621 (Human); 107250 (Mouse); 293997 (Rat)

Gene Symbol

KAZALD1

UniProt

Q96I82

Additional IGFBP-rP10 Products

Product Documents for Human IGFBP‑rP10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IGFBP‑rP10 Antibody

For research use only

Citations for Human IGFBP‑rP10 Antibody

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