Human IgG Fc Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB110V-025
FAB110V-100
Human IgG Fc Alexa Fluor® 405-conjugated Antibody in Data
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Human IgG Fc Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 97924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IgG<sub>1</sub>in HEK293 Human Cell Line Transfected with Human CD94 Fc Chimera by Flow Cytometry. View Larger

Detection of IgG1in HEK293 Human Cell Line Transfected with Human CD94 Fc Chimera by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either human CD94 Fc Chimera (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human IgG Fc Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB110V). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG

Product Datasheets

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