Human IgG Fc PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB110P-025
FAB110P-100
Detection of IgG1in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.
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Product Details
Citations (2)
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Reviews (1)

Human IgG Fc PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 97924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IgG<sub>1</sub>in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of IgG1in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP either (A) bound to Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) or (B) untreated was stained with Mouse Anti-Human IgG PE-conjugated Monoclonal Antibody (Catalog # FAB110P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

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Citations for Human IgG Fc PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Pressure increases PD-L1 expression in A549 lung adenocarcinoma cells and causes resistance to anti-ROR1 CAR T cell-mediated cytotoxicity
    Authors: Z Ou, X Dou, N Tang, G Liu
    Scientific Reports, 2022-04-28;12(1):6919.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by�epistasis acting on ACE2
    Authors: GM Castiglion, L Zhou, Z Xu, Z Neiman, CF Hung, EJ Duh
    PloS Biology, 2021-12-21;19(12):e3001510.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. What is the light chain of the Human IgG Fc Antibody, Catalog #s MAB110 and FAB110, Clone # 97924?

    • Catalog #s MAB110 and FAB110 have a kappa light chain.

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Reviews for Human IgG Fc PE-conjugated Antibody

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Human IgG Fc PE-conjugated Antibody
By Anonymous on 10/06/2020
Application: Flow Sample Tested: 293T human embryonic kidney cell line,HEK293T human embryonic kidney cell line Species: Human