Human IgG Fc PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB110P-025
FAB110P-100

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Detection of IgG1in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.
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Citations (2)
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Reviews (1)

Human IgG Fc PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 97924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IgG<sub>1</sub>in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of IgG1in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP either (A) bound to Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) or (B) untreated was stained with Mouse Anti-Human IgG PE-conjugated Monoclonal Antibody (Catalog # FAB110P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

Human IgG Fc PE‑conjugated Antibody Monoclonal Mouse IgG1 Clone # 97924

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

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Citations for Human IgG Fc PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Pressure increases PD-L1 expression in A549 lung adenocarcinoma cells and causes resistance to anti-ROR1 CAR T cell-mediated cytotoxicity
    Authors: Z Ou, X Dou, N Tang, G Liu
    Scientific Reports, 2022-04-28;12(1):6919.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by�epistasis acting on ACE2
    Authors: GM Castiglion, L Zhou, Z Xu, Z Neiman, CF Hung, EJ Duh
    PloS Biology, 2021-12-21;19(12):e3001510.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. What is the light chain of the Human IgG Fc Antibody, Catalog #s MAB110 and FAB110, Clone # 97924?

    • Catalog #s MAB110 and FAB110 have a kappa light chain.

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Reviews for Human IgG Fc PE-conjugated Antibody

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Human IgG Fc PE-conjugated Antibody
By Anonymous on 10/06/2020
Application: Flow Sample Tested: 293T human embryonic kidney cell line,HEK293T human embryonic kidney cell line Species: Human