|Detection of IgG1 in HEK293 Human Cell Line Transfected with Human CD94 Fc Chimera by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either human CD94 Fc Chimera (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human IgG Fc Biotinylated Monoclonal Antibody (Catalog # FAB110B), followed by Streptavidin-Allophycocyanin (Catalog # F0050). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.