Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 97924
View all IgG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region

Specificity

Detects chimeric proteins containing a human IgG Fc region in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IgG Fc Biotinylated Antibody

Detection of IgG1in HEK293 Human Cell Line Transfected with Human Fc Chimera by Flow Cytometry.

Detection of IgG1in HEK293 Human Cell Line Transfected with Human Fc Chimera by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either human Fc Chimera (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human IgG Fc Biotinylated Monoclonal Antibody (Catalog # FAB110B), followed by Streptavidin-Allophycocyanin (F0050). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
Detection of IgG1 antibody in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP antibody by Flow Cytometry.

Detection of IgG1 in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP either (A) bound to Recombinant Human B7-H1/PD-L1 Fc Chimera (156-B7) or (B) untreated was stained with Mouse Anti-Human IgG biotin-conjugated Monoclonal Antibody (Catalog # FAB110B) followed by APC-conjugated Streptavidin (F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IgG Fc Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Fc Chimera was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) or Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

Human IgG Fc Biotinylated Antibody Monoclonal Mouse IgG1 Clone # 97924

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Human IgG Fc Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IgG Fc Biotinylated Antibody

For research use only

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FAQs for Human IgG Fc Biotinylated Antibody

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  • Q: What is the light chain of the Human IgG Fc Antibody, Catalog #s MAB110 and FAB110, Clone # 97924?

    A: Catalog #s MAB110 and FAB110 have a kappa light chain.

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