Human IgG Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB110R-100UG
Detection of IgG Fc in Mouse B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Mouse PD-1 and eGFP by Flow Cytometry.
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Human IgG Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 97924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IgG Fc antibody in Mouse B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Mouse PD-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of IgG Fc in Mouse B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Mouse PD-1 and eGFP by Flow Cytometry. (A) Recombinant Mouse B7-H1/PD-L1 Fc Chimera (Catalog # 1019-B7) bound to HEK293 human embryonic kidney cell line transfected with mouse PD-1 and eGFP or (B) cells alone, were stained with Mouse Anti-Human IgG Fc Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB110R). Quadrants were set based on Mouse IgG1 Alexa Fluor® 647 Isotype Control (Catalog # IC002R, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human IgG Alexa Fluor® 647-conjugated Antibody
By Anonymous on 01/20/2022
Application: Flow Sample Tested: primary cornea epithelial cells Species: primary cornea epithelial cells