Human IgG Alexa Fluor® 647-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IgG Fc in Mouse B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Mouse PD-1 and eGFP by Flow Cytometry. (A) Recombinant Mouse B7-H1/PD-L1 Fc Chimera (Catalog # 1019-B7) bound to HEK293 human embryonic kidney cell line transfected with mouse PD-1 and eGFP or (B) cells alone, were stained with Mouse Anti-Human IgG Fc Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB110R). Quadrants were set based on Mouse IgG1 Alexa Fluor® 647 Isotype Control (Catalog # IC002R, data not shown). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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